Gene Rv3378c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Produces isotuberculosinol (nosyberkol) from halimadienyl diphosphate. Production of tuberculosinol has also been shown by some. |
| Product | Diterpene synthase |
| Comments | Rv3378c, (MTV004.36c), len: 296 aa. Diterpene synthase. Note that this ORF and the downstream ORF MTV004.35c have a significantly lower GC bias than the rest of the genome. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). Cofactor: Mg2+. |
| Functional category | Intermediary metabolism and respiration |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). M. tuberculosis CDC1551 transposon mutant fails to prevent phagosome maturation and acidification in bone marrow macrophages from BALB/c mice; mutant shows reduced survival in these macrophages (See Pethe et al., 2004). M. tuberculosis CDC1551 Rv3377c-Rv3378c mutant does not produce halimadienyl diphosphate or nosyberkol (See Mann et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3792358 | 3793248 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3378c|Rv3378c
MNLVSEKEFLDLPLVSVAEIVRCRGPKVSVFPFDGTRRWFHLECNPQYDDYQQAALRQSIRILKMLFEHGIETVISPIFSDDLLDRGDRYIVQALEGMALLANDEEILSFYKEHEVHVLFYGDYKKRLPSTAQGAAVVKSFDDLTISTSSNTEHRLCFGVFGNDAAESVAQFSISWNETHGKPPTRREIIEGYYGEYVDKADMFIGFGRFSTFDFPLLSSGKTSLYFTVAPSYYMTETTLRRILYDHIYLRHFRPKPDYSAMSADQLNVLRNRYRAQPDRVFGVGCVHDGIWFAEG
Bibliography
- Pethe K et al. [2004]. Isolation of Mycobacterium tuberculosis mutants defective in the arrest of phagosome maturation. Mutant
- Becq J, Gutierrez MC, Rosas-Magallanes V, Rauzier J, Gicquel B, Neyrolles O and Deschavanne P [2007]. Contribution of horizontally acquired genomic islands to the evolution of the tubercle bacilli. Sequence
- Mann FM et al. [2009]. Edaxadiene: a new bioactive diterpene from Mycobacterium tuberculosis. Function Product
- Spangler JE et al. [2010]. Synthesis enables a structural revision of the Mycobacterium tuberculosis-produced diterpene, edaxadiene. Function
- Maugel N et al. [2010]. Synthesis of (+/-)-nosyberkol (isotuberculosinol, revised structure of edaxadiene) and (+/-)-tuberculosinol. Function
- Prach L et al. [2010]. Diterpene production in Mycobacterium tuberculosis. Localization
- Nakano C et al. [2011]. Characterization of the Rv3378c gene product, a new diterpene synthase for producing tuberculosinol and (13R, S)-isotuberculosinol (nosyberkol), from the Mycobacterium tuberculosis H37Rv genome. Function
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- Mann FM et al. [2011]. Magnesium depletion triggers production of an immune modulating diterpenoid in Mycobacterium tuberculosis. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
