Gene Rv3382c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown. Possibly involved in drug/antibiotic tolerance. In other organisms, LYTB product is involved in penicillin tolerance and control of the stringent response. |
| Product | Probable LYTB-related protein LytB1 |
| Comments | Rv3382c, (MTV004.40c), len: 329 aa. Probable lytB1, lytB-related protein, highly similar to many e.g. Q9HVM7|LYTB_PSEAE|PA4557 from Pseudomonas aeruginosa (314 aa), FASTA scores: opt: 1048, E(): 2e-55, (53.2% identity in 314 aa overlap); Q9JR39|LYTB|NMA0624|NMB1831 from Neisseria meningitidis (serogroup a and B) (322 aa), FASTA scores: opt: 1041, E(): 5.4e-55, (52.25% identity in 312 aa overlap); P22565|LYTB_ECOLI|B0029 from Escherichia coli strain K12 (316 aa), FASTA scores: opt: 1013, E(): 2.5e-53, (51.45% identity in 311 aa overlap) (for more information about lytB protein, see citation below); Q9X781|LYTB_MYCLE|LYTB2|ML1938|MLCB1222.06c from Mycobacterium leprae (332 aa), FASTA scores: opt: 979, E(): 2.8e-51, (51.3% identity in 312 aa overlap); etc. Also similar to Q9PAS9|XF2416 drug tolerance protein from Xylella fastidiosa (316 aa), FASTA scores: opt: 1043, E(): 4.1e-55, (53.65% identity in 315 aa overlap). And similar to O53458|Rv1110|LYTB2|MTV017.63 from Mycobacterium tuberculosis (335 aa), FASTA scores: opt: 975, E(): 4.9e-51, (51.3% identity in 312 aa overlap). Belongs to the LytB family. |
| Functional category | Cell wall and cell processes |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3796448 | 3797437 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3382c|lytB1
MAEVFVGPVAQGYASGEVTVLLASPRSFCAGVERAIETVKRVLDVAEGPVYVRKQIVHNTVVVAELRDRGAVFVEDLDEIPDPPPPGAVVVFSAHGVSPAVRAGADERGLQVVDATCPLVAKVHAEAARFAARGDTVVFIGHAGHEETEGTLGVAPRSTLLVQTPADVAALNLPEGTQLSYLTQTTLALDETADVIDALRARFPTLGQPPSEDICYATTNRQRALQSMVGECDVVLVIGSCNSSNSRRLVELAQRSGTPAYLIDGPDDIEPEWLSSVSTIGVTAGASAPPRLVGQVIDALRGYASITVVERSIATETVRFGLPKQVRAQ
Bibliography
- Gustafson CE et al. [1993]. Identification of the Escherichia coli lytB gene, which is involved in penicillin tolerance and control of the stringent response. Homolog Function
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
