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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
intermediary metabolism and respiration
regulatory proteins
conserved hypotheticals
lipid metabolism
General annotation
FunctionInvolved in carotenoid biosynthesis and in astaxanthin biosynthetic pathway. Catalyses the reaction from prephytoene diphosphate to phytoene [catalytic activity 1: 2 geranylgeranyl diphosphate = pyrophosphate + prephytoene diphosphate] [catalytic activity 2: prephytoene diphosphate = pyrophosphate + phytoene].
ProductProbable phytoene synthase PhyA
CommentsRv3397c, (MTCY78.31), len: 302 aa. Probable phyA (alternate gene name: crtB), phytoene synthase, similar to many others e.g. Q9X7V5|SC6A5.09 from Streptomyces coelicolor (312 aa), FASTA scores: opt: 791, E(): 2.8e-43, (48.25% identity in 286 aa overlap); Q9RW07|DR0862 from Deinococcus radiodurans (325 aa), FASTA scores: opt: 482, E(): 1.5e-23, (35.25% identity in 292 aa overlap); Q9JRU9|NMB1168|NMB1130 from Neisseria meningitidis (serogroup B) (290 aa), FASTA scores: opt: 446, E(): 2.8e-21, (34.25% identity in 260 aa overlap); P37272|PSY_CAPAN from Capsicum annuum (Bell pepper) (419 aa), FASTA scores: opt: 431, E(): 3.4e-20, (33.0% identity in 288 aa overlap); etc. Also similar to Q9JUF5|NMA1339 putative poly-isoprenyl transferase from Neisseria meningitidis (serogroup A) (290 aa), FASTA scores: opt: 450, E(): 1.6e-21, (34.6% identity in 260 aa overlap). And similar to crtB|O05424 phytoene synthase from Mycobacterium marinum (319 aa), blastp scores: 113, E= 6e-24, Identities = 89/283 (31%) (see citation below). Contains PS01045 Squalene and phytoene synthases signature 2. Belongs to the phytoene/squalene synthetase family.
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified in culture filtrates of M. tuberculosis H37Rv (See Malen et al., 2007).
MutantNon-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017).
Check for mutants available at TARGET website
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3397c|phyA