Gene Rv3397c (crtB)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in carotenoid biosynthesis and in astaxanthin biosynthetic pathway. Catalyses the reaction from prephytoene diphosphate to phytoene [catalytic activity 1: 2 geranylgeranyl diphosphate = pyrophosphate + prephytoene diphosphate] [catalytic activity 2: prephytoene diphosphate = pyrophosphate + phytoene]. |
| Product | Probable phytoene synthase PhyA |
| Comments | Rv3397c, (MTCY78.31), len: 302 aa. Probable phyA (alternate gene name: crtB), phytoene synthase, similar to many others e.g. Q9X7V5|SC6A5.09 from Streptomyces coelicolor (312 aa), FASTA scores: opt: 791, E(): 2.8e-43, (48.25% identity in 286 aa overlap); Q9RW07|DR0862 from Deinococcus radiodurans (325 aa), FASTA scores: opt: 482, E(): 1.5e-23, (35.25% identity in 292 aa overlap); Q9JRU9|NMB1168|NMB1130 from Neisseria meningitidis (serogroup B) (290 aa), FASTA scores: opt: 446, E(): 2.8e-21, (34.25% identity in 260 aa overlap); P37272|PSY_CAPAN from Capsicum annuum (Bell pepper) (419 aa), FASTA scores: opt: 431, E(): 3.4e-20, (33.0% identity in 288 aa overlap); etc. Also similar to Q9JUF5|NMA1339 putative poly-isoprenyl transferase from Neisseria meningitidis (serogroup A) (290 aa), FASTA scores: opt: 450, E(): 1.6e-21, (34.6% identity in 260 aa overlap). And similar to crtB|O05424 phytoene synthase from Mycobacterium marinum (319 aa), blastp scores: 113, E= 6e-24, Identities = 89/283 (31%) (see citation below). Contains PS01045 Squalene and phytoene synthases signature 2. Belongs to the phytoene/squalene synthetase family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in culture filtrates of M. tuberculosis H37Rv (See Malen et al., 2007). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3814090 | 3814998 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3397c|phyA
MTEIEQAYRITESITRTAARNFYYGIRLLPREKRAALSAVYALGRRIDDVADGELAPETKITELDAIRKSLDNIDDSSDPVLVALADAARRFPVPIAMFAELIDGARMEIDWTGCRDFDELIVYCRRGAGTIGKLCLSIFGPVSTATSRYAEQLGIALQQTNILRDVREDFLNGRIYLPRDELDRLGVRLRLDDTGALDDPDGRLAALLRFSADRAADWYSLGLRLIPHLDRRSAACCAAMSGIYRRQLALIRASPAVVYDRRISLSGLKKAQVAAAALASSVTCGPAHGPLPADLGSHPSH
Bibliography
- Ramakrishnan L et al. [1997]. A crtB homolog essential for photochromogenicity in Mycobacterium marinum: isolation, characterization, and gene disruption via homologous recombination. Homolog Mutant Function
- Målen H et al. [2007]. Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
