Gene Rv3401
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; probably enzyme involved in cellular metabolism. |
| Product | Conserved protein |
| Comments | Rv3401, (MTCY78.27c), len: 786 aa. Conserved protein, may be an hydrolase or a transferase, equivalent to Q49736|ML0392|B1620_F1_30 hypothetical 88.1 KDA protein from Mycobacterium leprae (792 aa), FASTA scores: opt: 4820, E(): 0, (91.45% identity in 782 aa overlap). Also highly similar to Q9L2I8|SCF42.31c putative glycosyl transferase from Streptomyces coelicolor (792 aa), FASTA scores: opt: 3060, E(): 2.9e-179, (59.25% identity in 785 aa overlap); and similar to others e.g. Q9K109|NMB0390 maltose phosphorylase from Neisseria meningitidis (serogroup B) (752 aa), FASTA scores: opt: 980, E(): 3.5e-52, (29.2% identity in 774 aa overlap); Q9JSW8|MAPA|NMA2098 putative maltose phosphorylase from Neisseria meningitidis (serogroup A) (752 aa), FASTA scores: opt: 956, E(): 1e-50, (28.4% identity in 764 aa overlap); O06993|YVDK_BACSU hypothetical 88.3 KDA protein (belongs to family 65 of glycosyl hydrolases) from Bacillus subtilis (757 aa), FASTA scores: opt: 926, E(): 6.9e-49, (28.5% identity in 754 aa overlap); Q9CF04|MAPA maltosephosphorylase from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (751 aa), FASTA scores: opt: 907, E(): 1e-47, (26.95% identity in 753 aa overlap); P77154|YCJT_ECOLI|B1316 hypothetical 84.9 KDA protein (belongs to family 65 of glycosyl hydrolases) from Escherichia coli strain K12 (755 aa), FASTA scores: opt: 392, E(): 2.9e-16, (27.5% identity in 774 aa overlap); etc. Also similar to Mycobacterium tuberculosis hypothetical protein Q10850|YK06_MYCTU|Rv2006|MT2062|MTCY39.11c (1327 aa), (27.2% identity in 802 aa overlap); note that Rv3400 and Rv3401 are similar to beginning and end of Q10850|YK06_MYCTU|Rv2006|MT2062|MTCY39.11c with approx. 270 aa missing from the middle. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by proteomics at the Statens Serum Institute (Denmark) (See Rosenkrands et al., 2000). Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the membrane fraction of M. tuberculosis H37Rv using nanoLC-MS/MS (See Xiong et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). Translational start site supported by proteomics data (See de Souza et al., 2011) (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3818042 | 3820402 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3401|Rv3401
MITEDAFPVEPWQVRETKLNLNLLAQSESLFALSNGHIGLRGNLDEGEPFGLPGTYLNSFYEIRPLPYAEAGYGYPEAGQTVVDVTNGKIFRLLVGDEPFDVRYGELISHERILDLRAGTLTRRAHWRSPAGKQVKVTSTRLVSLAHRSVAAIEYVVEAIEEFVRVTVQSELVTNEDVPETSADPRVSAILDRPLQAVEHERTERGALLMHRTRASALMMAAGMEHEVEVPGRVEITTDARPDLARTTVICGLRPGQKLRIVKYLAYGWSSLRSRPALRDQAAGALHGARYSGWQGLLDAQRAYLDDFWDSADVEVEGDPECQQAVRFGLFHLLQASARAERRAIPSKGLTGTGYDGHAFWDTEGFVLPVLTYTAPHAVADALRWRASTLDLAKERAAELGLEGAAFPWRTIRGQESSAYWPAGTAAWHINADIAMAFERYRIVTGDGSLEEECGLAVLIETARLWLSLGHHDRHGVWHLDGVTGPDEYTAVVRDNVFTNLMAAHNLHTAADACLRHPEAAEAMGVTTEEMAAWRDAADAANIPYDEELGVHQQCEGFTTLAEWDFEANTTYPLLLHEAYVRLYPAQVIKQADLVLAMQWQSHAFTPEQKARNVDYYERRMVRDSSLSACTQAVMCAEVGHLELAHDYAYEAALIDLRDLHRNTRDGLHMASLAGAWTALVVGFGGLRDDEGILSIDPQLPDGISRLRFRLRWRGFRLIVDANHTDVTFILGDGPGTQLTMRHAGQDLTLHTDTPSTIAVRTRKPLLPPPPQPPGREPVHRRALAR
Bibliography
- Rosenkrands I et al. [2000]. Towards the proteome of Mycobacterium tuberculosis. Proteomics
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Xiong Y, Chalmers MJ, Gao FP, Cross TA and Marshall AG [2005]. Identification of Mycobacterium tuberculosis H37Rv integral membrane proteins by one-dimensional gel electrophoresis and liquid chromatography electrospray ionization tandem mass spectrometry. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Proteogenomic analysis of polymorphisms and gene annotation divergences in prokaryotes using a clustered mass spectrometry-friendly database. Proteomics Sequence
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
