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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionThe sigma factor is an initiation factor that promotes attachment of the RNA polymerase to specific initiation sites and then is released.
ProductProbable alternative RNA polymerase sigma-D factor SigD
CommentsRv3414c, (MTCY78.15), len: 212 aa. Probable sigD, alternative RNA polymerase sigma-D factor (see citations below), similar to others (notably from Streptomyces coelicolor) e.g. Q9L0I8|SCD63.01 from Streptomyces coelicolor (195 aa), FASTA scores: opt: 533, E(): 9.6e-28, (47.25% identity in 182 aa overlap); Q9FDS3|ADSA from Streptomyces griseus (258 aa), FASTA scores: opt: 223, E(): 1.8e-07, (28.95% identity in 183 aa overlap); BAB48649|MLL1224 from Rhizobium loti (Mesorhizobium loti) (187 aa), FASTA scores: opt: 202, E(): 3.2e-06, (30.4% identity in 194 aa overlap); P38133|RPOE_STRCO|SIGE|SCE94.07 from Streptomyces coelicolor (176 aa), FASTA scores: opt: 200, E(): 4.1e-06, (35.25% identity in 156 aa overlap); P37978|CNRH_ALCEU from Alcaligenes eutrophus (Ralstonia eutropha), FASTA scores: opt: 197, E(): 6.9e-06, (30.35% identity in 191 aa overlap); etc. C-terminus strongly similar to N-terminal part of Q49727|S1620B|B1620_C3_233 hypothetical 6.2 KDA protein from Mycobacterium leprae (59 aa), FASTA scores: opt: 217, E(): 1.3e-07, (90.25% identity in 41 aa overlap). Belongs to the sigma-70 factor family.
Functional categoryInformation pathways
TranscriptomicsmRNA identified by SCOTS method, during infection of cultured human primary macrophages (see Graham & Clark-Curtiss 1999). mRNA also identified by real-time quantitative RT-PCR during exponential growing cultures. mRNA level decreased after heat shock (see Manganelli et al., 1999).
MutantNon-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). M. tuberculosis H37Rv sigD|Rv3414c mutant is attenuated in BALB/c mice; growth in vitro, in hypoxic conditions, under oxidative stress (plumbagin, cumene hydroperoxide, diamide) is comparable to wild-type; slightly more susceptible to INH (See Raman et al., 2004).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS38330383833676-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3414c|sigD
MVDPGVSPGCVRFVTLEISPSMTMQGERLDAVVAEAVAGDRNALREVLETIRPIVVRYCRARVGTVERSGLSADDVAQEVCLATITALPRYRDRGRPFLAFLYGIAAHKVADAHRAAGRDRAYPAETLPERWSADAGPEQMAIEADSVTRMNELLEILPAKQREILILRVVVGLSAEETAAAVGSTTGAVRVAQHRALQRLKDEIVAAGDYA