Gene Rv3428c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in the transposition of the insertion sequence IS1532. |
| Product | Possible transposase |
| Comments | Rv3428c, (MTCY78.01, len: 410 aa. Possible transposase insertion sequence, similar to others e.g. Q9APG9|ORF1 from Pseudomonas putida (509 aa), FASTA scores: opt: 578, E(): 1.1e-29, (32.45% identity in 376 aa overlap); P55379|Y4BL_RHISN from Rhizobium sp. strain NGR234 (516 aa), FASTA scores: opt: 665, E(): 2.7e-35, (35.3% identity in 391 aa overlap); etc. |
| Functional category | Insertion seqs and phages |
| Proteomics | Identified in culture filtrates of M. tuberculosis H37Rv (See Malen et al., 2007). Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3844738 | 3845970 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3428c|Rv3428c
VATIAQRLRDDHGVAASESSVRRWIATHFAEEVARERVTVPRGPVDAGSEAQIDYGRLGMWFDPATARRVAVWAFVMVLAFSRHLFVRPVIRMDQTAWCACHVAAFEFFDGVPARLVCDNLRTGVDKPDLYDPQINRSYAELASHYATLVDPARARKPKDKPRVERPMTYVRDSFWKGREFDSLAQMQQAAVTWSTEVAGLRYLRALEGAQPLRMFEAVEQQALIALPPRAFELTSWSIGTVGVDTHLKVGKALYSVPWRLIGQRLHARTAGDVVQIFAGNDVVATHVRRPSGRSTDFSHYPPEKIAFHMRTPTWCRHTAELVGPASQQVIAEFMRDNAIHHLRSAQGVLGLRDKHGCDRLEAACARAIEVGDPSYRTIKGILVAGTEHAANEPTTSSPASTAGGVPARP
Bibliography
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2007]. Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
