Gene Rv3450c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown |
| Product | ESX conserved component EccB4. ESX-4 type VII secretion system protein. Probable membrane protein. |
| Comments | Rv3450c, (MTCY13E12.03c), len: 470 aa. EccB4, esx conserved component, ESX-4 type VII secretion system protein, probable membrane protein (possible membrane spanning region near N-terminus). Similar to hypothetical unknowns proteins from Mycobacterium leprae e.g. O33088|MLCB628.17C|ML0054 hypothetical 51.9 KDA protein (putative membrane protein)(481 aa), FASTA scores: opt: 708, E(): 6.4e-32, (32.9% identity in 480 aa overlap); Q9CD29|ML2536 (552 aa), FASTA scores: opt: 394, E(): 1.7e-14, (33.6% identity in 503 aa overlap); etc. Also similar to other proteins from Mycobacterium tuberculosis (strains H37Rv and CDC1551) e.g. O69734|Rv3869|MTV027.04 (480 aa), FASTA scores: opt: 717, E(): 2e-32, (32.55% identity in 479 aa overlap); O05449|Rv3895c|MTCY15F10.17 (495 aa), FASTA scores: opt: 670, E(): 8.3e-30, (36.4% identity in 475 aa overlap); O5368|Rv0283|MTV035.11 (538 aa), FASTA scores: opt: 467, E(): 1.5e-18, (36.3% identity in 493 aa overlap); etc. |
| Functional category | Cell wall and cell processes |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3871084 | 3872496 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3450c|eccB4
VPSPATTWLHVSGYRFLLRRIECALLFGDVCAATGALRARTTSLALGCVLAIVAAMGCAFVALLRPQSALGQAPIVMGRESGALYVRVDDVWHPVLNLASARLIAATNANPQPVSESELGHTKRGPLLGIPGAPQLLDQPLAGAESAWAICDSDNGGSTTVVVGPAEDSSAQVLTAEQMILVATESGSPTYLLYGGRRAVVDLADPAVVWALRLQGRVPHVVAQSLLNAVPEAPRITAPRIRGGGRASVGLPGFLVGGVVRITRASGDEYYVVLEDGVQRIGQVAADLLRFGDSQGSVNVPTVAPDVIRVAPIVNTLPVSAFPDRPPTPVDGSPGRAVTTLCVTWTPAQPGAARVAFLAGSGPPVPLGGVPVTLAQADGRGPALDAVYLPPGRSAYVAARSLSGGGTGTRYLVTDTGVRFAIHDDDVAHDLGLPTAAIPAPWPVLATLPSGPELSRANASVARDTVAPGP
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- [2009]. Systematic genetic nomenclature for type VII secretion systems. Nomenclature
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
