Gene Rv3469c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in aromatic hydrocarbons degradation [catalytic activity: 4-hydroxy-2-oxovalerate = pyruvate + acetaldehyde]. |
| Product | Probable 4-hydroxy-2-oxovalerate aldolase MhpE (HOA) |
| Comments | Rv3469c, (MTCY13E12.22c), len: 336 aa. Probable mhpE, 4-hydroxy-2-oxovalerate aldolase, similar to others (principally from Pseudomonas species) e.g. Q99PZ1|SCP1.301|SCP1.53c from Streptomyces coelicolor (338 aa), FASTA scores: opt: 615, E(): 7.9e-31, (37.65% identity in 332 aa overlap); Q9X9Q0|NIKB NIKB protein (see Bruntner et al., 1999) from Streptomyces tendae (357 aa), FASTA scores: opt: 571, E(): 4.4e-28, (34.5% identity in 339 aa overlap); P51014|BPHF_PSES1 from Pseudomonas sp. strain KKS102 (352 aa), FASTA scores: opt: 549, E(): 9.9e-27, (31.2% identity in 314 aa overlap); Q51983|CMTG_PSEPU from Pseudomonas putida (350 aa), FASTA scores: opt: 543, E(): 2.3e-26, (30.7% identity in 319 aa overlap); P51020|MHPE_ECOLI|MHPF|B0352 from Escherichia coli strain K12 (337 aa), FASTA scores: opt: 517, E(): 9.1e-25, (31.75% identity in 312 aa overlap); etc. Also similar to P71867|MTCY03C7.22|Rv3534c hypothetical 36.4 KDA protein from Mycobacterium tuberculosis (346 aa), FASTA scores: E(): 7.5e-24, (31.9% identity in 310 aa overlap). |
| Functional category | Intermediary metabolism and respiration |
| Transcriptomics | mRNA identified by microarray analysis; transcription repressed at low pH in vitro conditions, which may mimic an environmental signal encountered by phagocytosed bacteria (see Fisher et al., 2002). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3886073 | 3887083 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3469c|mhpE
MLMTATHREPIVLDTTVRDGSYAVNFQYTDDDVRRIVGDLDAAGIPYIEIGHGVTIGAAAAQGPAAHTDEEYFRAARSVVRNARLGAVIVPALARIETVDLAGDYLDFLRICVIATEFELVMPFVERAQSKGLEVSIQLVKSHLFEPDVLAAAGKRARDVGVRIVYVVDTTGTFLPEDARRYVEALRGASDVSVGFHGHNNLAMAVANTLEAFDAGADFLDGTLMGFGRGAGNCQIECLVAALQRRGHLAAVDLDRIFDAARSDMLGRSPQSYGIDPWEISFGFHGLDSLQVEHLRAAAQQAGLSVSHVIRQTAKSHAGQWLSPQDIDRVVVGMRA
Bibliography
- Bruntner C et al. [1999]. Molecular characterization of co-transcribed genes from Streptomyces tendae Tü901 involved in the biosynthesis of the peptidyl moiety of the peptidyl nucleoside antibiotic nikkomycin. Homolog Sequence Product Mutant
- Fisher MA, Plikaytis BB and Shinnick TM [2002]. Microarray analysis of the Mycobacterium tuberculosis transcriptional response to the acidic conditions found in phagosomes. Transcriptome Regulation
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
