Gene Rv3480c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | May be involved in synthesis of triacylglycerol |
| Product | Possible triacylglycerol synthase (diacylglycerol acyltransferase) |
| Comments | Rv3480c, (MTCY13E12.33c), len: 497 aa. Possible triacylglycerol synthase (See Daniel et al., 2004), similar to many from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. O69701|Y1D4_MYCTU|Rv3734c|MT3839|MTV025.082c (454 aa), FASTA scores: opt: 520, E(): 2e-23, (39.95% identity in 488 aa overlap); Q10554|Y895_MYCTU|Rv0895|MTCY31.23 (505 aa), FASTA scores: opt: 434, E(): 2.7e-18, (34.2% identity in 497 aa overlap); AAK45165|MT0919 (520 aa), FASTA scores: opt: 434, E(): 2.7e-18, (34.2% identity in 497 aa overlap); etc. Also similar to Q9X7A8|MLCB1610.05|ML1244 conserved membrane protein from Mycobacterium leprae (491 aa), FASTA scores: opt: 272, E(): 1e-08, (28.85% identity in 485 aa overlap); and Q9RIU8|CM11.13c hypothetical 47.1 KDA protein from Streptomyces coelicolor (446 aa), FASTA scores: opt: 254, E(): 1.1e-07, (30.4% identity in 497 aa overlap). Seems to belong to the UPF0089 family. |
| Functional category | Lipid metabolism |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Required for survival in primary murine macrophages, by transposon site hybridization (TraSH) in H37Rv (See Rengarajan et al., 2005). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3898909 | 3900402 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3480c|Rv3480c
VSQTARRLGPQDMFFLYSESSTTMMHVGALMPFTPPSGAPPDLLRQLVDESKASEVVEPWSLRLSHPELLYHPTQSWVVDDNFDLDYHVRRSALASPGDERELGIPVSRLHSHALDLRRPPWEVHFIEGLEGGRFAIYIKMHHSLIDGYTGQKMLARSLSTDPHDTTHPLFFNIPTPGRSPADTQDSVGGGLIAGAGNVLDGLGDVVRGLGGLVSGVGSVLGSVAGAGRSTFELTKALVNAQLRSDHEYRNLVGSVQAPHCILNTRISRNRRFATQQYPLDRLKAIGAQYDATINDVALAIIGGGLRRFLDELGELPNKSLIVVLPVNVRPKDDEGGGNAVATILATLGTDVADPVQRLAAVTASTRAAKAQLRSMDKDAILAYSAALMAPYGVQLASTLSGVKPPWPYTFNLCVSNVPGPEDVLYLRGSRMEASYPVSLVAHSQALNVTLQSYAGTLNFGFIGCRDTLPHLQRLAVYTGEALDQLAAADGAAGLGS
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Daniel J, Deb C, Dubey VS, Sirakova TD, Abomoelak B, Morbidoni HR and Kolattukudy PE [2004]. Induction of a novel class of diacylglycerol acyltransferases and triacylglycerol accumulation in Mycobacterium tuberculosis as it goes into a dormancy-like state in culture. Function Product
- Rengarajan J et al. [2005]. Genome-wide requirements for Mycobacterium tuberculosis adaptation and survival in macrophages. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
