Gene Rv3484
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown |
| Product | Possible conserved protein CpsA |
| Comments | Rv3484, (MTCY13E12.37), len: 512 aa. Possible cpsA, hypothetical protein, equivalent to Q50160|CPSA|ML2247 hypothetical protein CPSA from Mycobacterium leprae (516 aa), FASTA scores: opt: 2557, E(): 1.6e-143, (74.9% identity in 518 aa overlap); and with good similarity to Q9CCK9|ML0750 hypothetical protein from Mycobacterium leprae (489 aa), FASTA scores: opt: 855, E(): 4.6e-43, (34.45% identity in 502 aa overlap). Also similar (or with similarity) to hypothetical proteins from Mycobacterium tuberculosis: P96872|Rv3267|MTCY71.07 (498 aa), FASTA scores: opt: 928, E(): 2.3e-47, (37.35% identity in 498 aa overlap); and O53834|Rv0822c|MTV043.14c (684 aa), FASTA scores: opt: 425, E(): 1.5e-17, (26.15% identity in 524 aa overlap). Shows also similarity with various bacterial proteins e.g. Q9KZK0|SCE34.26 conserved hypothetical protein from Streptomyces coelicolor (507 aa), FASTA scores: opt: 329, E(): 5.3e-12, (28.85% identity in 478 aa overlap); Q9K4E6|2SC6G5.02 conserved hypothetical protein, possible membrane protein, from Streptomyces coelicolor (382 aa), FASTA scores: opt: 305, E(): 1.1e-10, (29.8% identity in 386 aa overlap); O69850|SC1C3.08c putative transcriptional regulator from Streptomyces coelicolor (366 aa), FASTA scores: opt: 304, E(): 1.2e-10, (29.6% identity in 395 aa overlap); Q9KZK3|SCE34.23 putative transcriptional regulator from Streptomyces coelicolor (396 aa), FASTA scores: opt: 296, E(): 3.8e-10, (31.25% identity in 349 aa overlap); AAK43602|CPSA CPSA protein from Streptococcus agalactiae (485 aa), FASTA scores: opt: 250, E(): 2.4e-07, (30.25% identity in 162 aa overlap); etc. Predicted to be an outer membrane protein (See Song et al., 2008). |
| Functional category | Conserved hypotheticals |
| Proteomics | Predicted secreted protein - identified in culture filtrates of M. tuberculosis H37Rv; signal peptide predicted (See Malen et al., 2007). Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate and membrane protein fraction of M. tuberculosis H37Rv but not whole cell lysates (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3903078 | 3904616 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3484|cpsA
MARSEGNRPRHRAVPQPSRIRKRLSRGVMTLVSVVALLMTGAGYWVAHGALGGITISQALTPEDPRSSGNNMNILLIGLDSRKDQEGNDLPWSVLKQLHAGDSDDGGYNTNTLILVHVGADGKVVAFSIPRDDWVPFTGVPGYNHIKIKEAYGLTKQYVAEQLANQGVSDRKELETRGREAARAATLRAVRSLTGVPIDYFAEINLAGFYDLAQTLGGVDVCLNHAVYDSYSGADFPAGRQRLNAAQALAFVRQRHGLDNGDLDRTHRQQAFLSSVMRELQDSGTFTNLDRLDNLMAVARKDVVLSAGWDEDLFRRMGDLAGGNVEFRTLPVVRYDNIDGQDVNIIDPTAIRAEVAAAFGSAPPTSQTAAAAKPNPSTVVDVVNAGSISGLASQVSGALLKRGYTAGQVRDRESGDPFTTAIEYGAGAETDAQNVADLLGIDAPNHPDPAVAPGHIRVTVDTNFSLPAPDEATAAATSTETSTYPLYGGGTTTDPTPDQGAPIDGGGVPCVN
Bibliography
- Sassetti CM and Rubin EJ [2003]. Genetic requirements for mycobacterial survival during infection. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2007]. Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv. Proteomics
- Song H, Sandie R, Wang Y, Andrade-Navarro MA and Niederweis M [2008]. Identification of outer membrane proteins of Mycobacterium tuberculosis. Localization
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
