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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionUnknown, but thought to be involved in host cell invasion. Predicted to be involved in lipid catabolism.
ProductPossible Mce-family lipoprotein LprN (Mce-family lipoprotein Mce4E)
CommentsRv3495c, (MTV023.02c), len: 384 aa. Possible lprN (alternate gene name: mce4E), lipoprotein which belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below), highly similar to Mycobacterium tuberculosis proteins O07417|LPRK|Rv0173|MTCI28.13|mce1E (390 aa); O07785|LPRL|Rv0593|MTCY19H5.29|mce2E (402 aa); and O53971|LPRM|Rv1970|MTV051.08|mce3E (377 aa). Also similar to others e.g. Q9F360|SC8A2.03c putative secreted protein from Streptomyces coelicolor (413 aa), FASTA scores: opt: 656, E(): 2.2e-32, (37.55% identity in 317 aa overlap); Q9CD10|LPRK|ML2593 putative lipoprotein from Mycobacterium leprae (392 aa), FASTA scores: opt: 616, E(): 5.5e-30, (28.95% identity in 373 aa overlap); etc. Contains possible signal sequence and appropriately positioned PS00013 Prokaryotic membrane lipoprotein lipid attachment site.
Functional categoryCell wall and cell processes
ProteomicsPredicted surface lipoprotein - identified in culture filtrates of M. tuberculosis H37Rv; signal peptide predicted (See Malen et al., 2007). Identified in the cell wall fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011).
MutantNon-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Non-essential gene for in vitro growth of H37Rv, but essential for in vitro growth on cholesterol; by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS39133803914534-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3495c|lprN
MNRIWLRAIILTASSALLAGCQFGGLNSLPLPGTAGHGEGAYSVTVEMADVATLPQNSPVMVDDVTVGSVAGIVAVQRPDGSFYAAVKLDLDKNVLLPANAVAKVSQTSLLGSLHVELAPPTDRPPTGRLVDGSRITEANTDRFPTTEEVFSALGVVVNKGNVGALEEIIDETHQAVAGRQAQFVNLVPRLAELTAGLNRQVHDIIDALDGLNRVSAILARDKDNLGRALDTLPDAVRVLNQNRDHIVDAFAALKRLTMVTSHVLAETKVDFGEDLKDLYSIVKALNDDRKDFVTSLQLLLTFPFPNFGIKQAVRGDYLNVFTTFDLTLRRIGETFFTTAYFDPNMAHMDEILNPPDFLIGELANLSGQAADPFKIPPGTASGQ
      
Bibliography