Gene Rv3502c (hsd4A)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; supposed involvement in cellular metabolism. Predicted to be involved in lipid catabolism. |
| Product | Probable short-chain type dehydrogenase/reductase. Possible 17-beta-hydroxysteroid dehydrogenase. |
| Comments | Rv3502c, (MTV023.09c), len: 317 aa (start uncertain). Probable short-chain dehydrogenase/reductase , similar to Mycobacterium tuberculosis proteins P71853|Rv3548c|MTCY03C7.08 hypothetical 31.1 KDA protein (304 aa), FASTA scores: opt: 739, E(): 6.2e-35, (45.15% identity in 310 aa overlap); and Q11020|YD50_MYCTU|FABG2|Rv1350|MT1393|MTCY02B10.14 putative oxidoreductase (247 aa), FASTA scores: opt: 475, E(): 5.1e-20, (40.15% identity in 254 aa overlap). Also similar to various dehydrogenases e.g. Q9I4V1|PA1023 probable short-chain dehydrogenase from Pseudomonas aeruginosa (305 aa), FASTA scores: opt: 535, E(): 2.3e-23, (37.1% identity in 302 aa overlap); Q9UVH9|FOX2 FOX2 protein (SDR family) (1015 aa), FASTA scores: opt: 487, E(): 3.2e-20, (38.4% identity in 276 aa overlap); P22414|FOX2_CANTR peroxisomal hydratase-dehydrogenase, D-3-hydroxyacyl CoA dehydrogenase (SDR family) from Candida tropicalis (Yeast) (906 aa) FASTA scores: opt: 481, E(): 6.4e-20, (38.0% identity in 250 aa overlap); P50171|DHB8_MOUSE|HSD17B8|HKE6|H2-KE6 estradiol 17 beta-dehydrogenase 8 from Mus musculus (Mouse) (260 aa) FASTA scores: opt: 459, E(): 4.3e-19, (39.75% identity in 259 aa overlap); CAC41362|BKR1 3-oxyacyl-[acyl-carrier protein] reductase (fragment) from Brassica napus (Rape) (317 aa), FASTA scores: opt: 447, E(): 2.4e-18, (39.2% identity in 255 aa overlap); etc. Contains PS00061 Short-chain dehydrogenases/reductases family signature. Belongs to the short-chain dehydrogenases/reductases (SDR) family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Non-essential gene for in vitro growth of H37Rv, but essential for in vitro growth on cholesterol; by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3921087 | 3922040 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3502c|Rv3502c
LKLTESNRSPRTTNTTDLSGKVAVVTGAAAGLGRAEALGLARLGATVVVNDVASALDASDVVDEIGAAAADAGAKAVAVAGDISQRATADELLASAVGLGGLDIVVNNAGITRDRMLFNMSDEEWDAVIAVHLRGHFLLTRNAAAYWRDKAKDAEGGSVFGRLVNTSSEAGLVGPVGQANYAAAKAGITALTLSAARALGRYGVCANVICPRARTAMTADVFGAAPDVEAGQIDPLSPQHVVSLVQFLASPAAAEVNGQVFIVYGPQVTLVSPPHMERRFSADGTSWDPTELTATLRDYFAGRDPEQSFSATDLMRQ
Bibliography
- Sassetti CM and Rubin EJ [2003]. Genetic requirements for mycobacterial survival during infection. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Kendall SL, Withers M, Soffair CN, Moreland NJ, Gurcha S, Sidders B, Frita R, Ten Bokum A, Besra GS, Lott JS and Stoker NG [2007]. A highly conserved transcriptional repressor controls a large regulon involved in lipid degradation in Mycobacterium smegmatis and Mycobacterium tuberculosis. Regulation
- Van der Geize R et al. [2007]. A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages. Function Product
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
