Gene Rv3517
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved hypothetical protein |
| Comments | Rv3517, (MTV023.24), len: 279 aa. Hypothetical protein, similar to several hypothetical mycobacterial proteins e.g. P71763|Rv1482c|MTCY277.03c from Mycobacterium tuberculosis strain H37Rv (339 aa) (alias AAK45794|MT1529 from Mycobacterium tuberculosis strain CDC1551 (292 aa) but longer) FASTA scores: opt: 1040, E(): 3.7e-60, (59.0% identity in 273 aa overlap); O07396|MAV346 from Mycobacterium avium (346 aa) FASTA scores: opt: 1018, E(): 1e-58, (57.2% identity in 278 aa overlap); O53421|Rv1073|MTV017.26 from Mycobacterium tuberculosis strain H37Rv (283 aa), FASTA scores: opt: 903, E(): 2.4e-51, (48.0% identity in 277 aa overlap); Q50134|U650AG|MLCB57.67c from Mycobacterium leprae (75 aa) FASTA scores: opt: 158, E(): 0.0015, (41.8% identity in 55 aa overlap); etc. |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified in the cytosol of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Operon | Rv3516 and Rv3517 are co-transcribed, by RT-PCR (see Roback et al., 2007). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3953431 | 3954270 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3517|Rv3517
VIEPFLGSEAIASGALTRHRLRSAYATIHPDVYVSPGADLTAWSRAQAAWLWSRRRGVIAGQSAAAMHGAKWVDARQAAELLYDHRRPPAGIHTWSDRVADDEIQPISGMNTTTPARTALDLARRYPVGKAVAAIDALARATDLKLADVEMLAERYRGSRGIRNARIALDLVDPGAESPRETWLRLLLIRAGFPRPQTQIPVYDEYGQLVAVIDMGWAGIKVGVDYEGDHHRTDRRTFNKDIKRAEALTELGWTDVRVTVEDTEGGIIWRVSAAWQRRT
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Roback P et al. [2007]. A predicted operon map for Mycobacterium tuberculosis. Operon
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
