Gene Rv3522
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; probably involved in lipid metabolism. |
| Product | Possible lipid transfer protein or keto acyl-CoA thiolase Ltp4 |
| Comments | Rv3522, (MTV023.29), len: 354 aa. Possible ltp4, lipid carrier protein or keto acyl-CoA thiolase, similar to several e.g. O30103|AF0134 3-ketoacyl-CoA thiolase (ACAB-4) from Archaeoglobus fulgidus (398 aa) FASTA scores: opt: 352, E(): 5.3e-15, (30.45% identity in 381 aa overlap); O29295|AF0967 3-ketoacyl-CoA thiolase (ACAB-9) from Archaeoglobus fulgidus (400 aa) FASTA scores: opt: 312, E(): 1.8e-12, (28.05% identity in 367 aa overlap); O29294|AF0968 3-ketoacyl-CoA thiolase (ACAB-10) from Archaeoglobus fulgidus (388 aa), FASTA scores: opt: 293, E(): 2.9e-11, (25.9% identity in 309 aa overlap); O58409|PH0676 long hypothetical nonspecific lipid-transfer protein (acethyl CoA synthetase) from Pyrococcus horikoshii (389 aa), FASTA scores: opt: 292, E(): 3.3e-11, (25.8% identity in 368 aa overlap); Q9Y9A3|APE2382 long hypothetical non specific lipid-transfer protein from Aeropyrum pernix (360 aa) FASTA scores: opt: 270, E(): 7.8e-10, (27.25% identity in 363 aa overlap); Q9YDI4|APE0929 long hypothetical nonspecific lipid-transfer protein from Aeropyrum pernix (400 aa), FASTA scores: opt: 258, E(): 4.9e-09, (26.45% identity in 306 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). |
| Functional category | Lipid metabolism |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3958448 | 3959512 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3522|ltp4
MSVRDIAVVGFAHAPHVRRTDGTTNGVEMLMPCFAQLYDELGITKADIGFWCSGSSDYLAGRAFSFISAIDSIGAVPPINESHVEMDAAWALYEAYIKLLTGEVDTALVYGFGKSSAGTLRRVLSRQTDPYTVAPLWPDSVSMAGLQARLGLDSGKWTHEQMARVAFDSFTNARRVDSVEPPITVGELLARPFFADPLRRHDIAPITDGAAAVVLAADNRARELRENPAWITGIEHRIESPALGARDITESPSTKLAAKIATGGHTGDIDVAEIHGPFTHQHLIVAEAIRIPGKTKVNPSGGPLAANPMFAAGLERIGFAAQHTWDGSARRVLAHATSGPALQQNLVAVMEGRG
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Kendall SL, Withers M, Soffair CN, Moreland NJ, Gurcha S, Sidders B, Frita R, Ten Bokum A, Besra GS, Lott JS and Stoker NG [2007]. A highly conserved transcriptional repressor controls a large regulon involved in lipid degradation in Mycobacterium smegmatis and Mycobacterium tuberculosis. Regulation
- Van der Geize R et al. [2007]. A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages. Function
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
