Gene Rv3526
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Predicted to be involved in lipid catabolism |
| Product | Oxygenase component of 3-ketosteroid-9-alpha-hydroxylase KshA |
| Comments | Rv3526, (MTCY03C7.30c), len: 386 aa. kshA, oxygenase component of 3-ketosteroid-9-alpha-hydroxylase, highly similar, except in C-terminus (also longer 69 aa), to O69348|ORF12 protein (function unknown) from Rhodococcus erythropolis (316 aa) FASTA scores: opt: 1137, E(): 6.9e-65, (59.6% identity in 250 aa overlap). Also some similarity with several aminopyrrolnitrin oxidases (PRND proteins, involved in the pathway for pyrrolnitrin biosynthesis, a secondary metabolite derived from tryptophan which has strong anti-fungal activity) e.g. Q9RPG0|PRND from Myxococcus fulvus (379 aa), FASTA scores: opt: 322, E(): 4.4e-13, (25.85% identity in 352 aa overlap); Q9RPG4|PRND from Burkholderia cepacia (Pseudomonas cepacia) (373 aa) FASTA scores: opt: 306, E(): 4.5e-12, (25.2% identity in 373 aa overlap); P95483|PRND from Pseudomonas fluorescens (363 aa), FASTA scores: opt: 305, E(): 5.1e-12, (25.0% identity in 372 aa overlap); etc. And also some similarity to other putative enzymes like dioxygenases, oxidases, vanillate O-demethyl oxygenase, etc. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Transcriptomics | mRNA identified by microarray analysis and up-regulated after 4h of starvation (see citation below). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, but essential for in vitro growth on cholesterol; by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011). M. tuberculosis H37Rv kshA|Rv3526 mutant shows in vitro growth defect with cholesterol, 5-alpha-androstane-3,17-dione, 4-androstene-3,17-dione as carbon source; mutant shows growth defect in BALB/c mice and in resting and activated J774A.1 and BALB/c bone marrow-derived macrophages; SCID mice infected with mutant survive longer than those infected with wild-type (See Hu et al., 2010). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3962439 | 3963599 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3526|kshA
VSTDTSGVGVREIDAGALPTRYARGWHCLGVAKDYLEGKPHGVEAFGTKLVVFADSHGDLKVLDGYCRHMGGDLSEGTVKGDEVACPFHDWRWGGDGRCKLVPYARRTPRMARTRSWTTDVRSGLLFVWHDHEGNPPDPAVRIPEIPEAASDEWTDWRWNRILIEGSNCRDIIDNVTDMAHFFYIHFGLPTYFKNVFEGHIASQYLHNVGRPDVDDLGTSYGEAHLDSEASYFGPSFMINWLHNRYGNYKSESILINCHYPVTQNSFVLQWGVIVEKPKGMSEEMTDKLSRVFTEGVSKGFLQDVEIWKHKTRIDNPLLVEEDGAVYQLRRWYEQFYVDVADIKPEMVERFEIEVDTKRANEFWNAEVEKNLKSREVSDDVPAEQH
Bibliography
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Dahl JL et al. [2003]. The role of RelMtb-mediated adaptation to stationary phase in long-term persistence of Mycobacterium tuberculosis in mice. Regulon
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Kendall SL, Withers M, Soffair CN, Moreland NJ, Gurcha S, Sidders B, Frita R, Ten Bokum A, Besra GS, Lott JS and Stoker NG [2007]. A highly conserved transcriptional repressor controls a large regulon involved in lipid degradation in Mycobacterium smegmatis and Mycobacterium tuberculosis. Regulation
- Van der Geize R et al. [2007]. A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages. Function Product
- Capyk JK et al. [2009]. Characterization of 3-ketosteroid 9{alpha}-hydroxylase, a Rieske oxygenase in the cholesterol degradation pathway of Mycobacterium tuberculosis. Biochemistry Structure
- Hu Y et al. [2010]. 3-Ketosteroid 9alpha-hydroxylase is an essential factor in the pathogenesis of Mycobacterium tuberculosis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
