Gene Mb3556
in Mycobacterium bovis AF2122/97
General annotation
| Type | CDS |
| Function | Unknown |
| Product | oxygenase component of 3-ketosteroid-9-alpha-hydroxylase ksha |
| Comments | Mb3556, -, len: 386 aa. Equivalent to Rv3526, len: 386 aa, from Mycobacterium tuberculosis strain H37Rv,(100.0% identity in 386 aa overlap). Hypothetical oxidoreductase (EC 1.-.-.-), highly similar, except in C-terminus (also longer 69 aa), to O69348|ORF12 PROTEIN (function unknown) from Rhodococcus erythropolis (316 aa) FASTA scores: opt: 1137, E(): 6.9e-65, (59.6% identity in 250 aa overlap). Also some similarity with several aminopyrrolnitrin oxidases (PRND proteins, involved in the pathway for pyrrolnitrin biosynthesis, a secondary metabolite derived from tryptophan which has strong anti-fungal activity) e.g. Q9RPG0|PRND from Myxococcus fulvus (379 aa), FASTA scores: opt: 322, E(): 4.4e-13,(25.85% identity in 352 aa overlap); Q9RPG4|PRND from Burkholderia cepacia (Pseudomonas cepacia) (373 aa) FASTA scores: opt: 306, E(): 4.5e-12, (25.2% identity in 373 aa overlap); P95483|PRND from Pseudomonas fluorescens (363 aa), FASTA scores: opt: 305, E(): 5.1e-12, (25.0% identity in 372 aa overlap); etc. And also some similarity to other putative enzymes like dioxygenases, oxidases, vanillate O-demethyl oxygenase, etc. |
| Functional category | Intermediary metabolism and respiration |
| Mutant | Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3905654 | 3906814 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium bovis AF2122-97|Mb3556|ksha
MSTDTSGVGVREIDAGALPTRYARGWHCLGVAKDYLEGKPHGVEAFGTKLVVFADSHGDLKVLDGYCRHMGGDLSEGTVKGDEVACPFHDWRWGGDGRCKLVPYARRTPRMARTRSWTTDVRSGLLFVWHDHEGNPPDPAVRIPEIPEAASDEWTDWRWNRILIEGSNCRDIIDNVTDMAHFFYIHFGLPTYFKNVFEGHIASQYLHNVGRPDVDDLGTSYGEAHLDSEASYFGPSFMINWLHNRYGNYKSESILINCHYPVTQNSFVLQWGVIVEKPKGMSEEMTDKLSRVFTEGVSKGFLQDVEIWKHKTRIDNPLLVEEDGAVYQLRRWYEQFYVDVADIKPEMVERFEIEVDTKRANEFWNAEVEKNLKSREVSDDVPAEQH
Bibliography
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