Gene Rv3551
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown. Probable subunit of a CoA-transferase composed of Rv3551|MTCY03C7.05c and Rv3552|MTCY03C7.03c. |
| Product | Possible CoA-transferase (alpha subunit) |
| Comments | Rv3551, (MTCY03C7.05c), len: 292 aa. Possible CoA-transferase, alpha subunit, similar in part to other CoA-transferases e.g. Q59111|GCTA_ACIFE|GCTA glutaconate CoA-transferase subunit A (GCT large subunit) from Acidaminococcus fermentans (319 aa) FASTA scores: opt: 247, E(): 6.3e-09, (27.35% identity in 307 aa overlap); Q9XD83|PCAI from Streptomyces sp. 2065 (251 aa), FASTA scores: opt: 222, E(): 2.3e-07, (27.55% identity in 243 aa overlap); BAB50895|MLL4183 from Rhizobium loti (Mesorhizobium loti) (285 aa), FASTA scores: opt: 206, E(): 2.8e-06, (27.4% identity in 281 aa overlap); etc. Also some similarity with O06167|SCOA_MYCTU|RVv504c|MT2579|MTCY07A7.10c probable succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A from Mycobacterium tuberculosis (248 aa), FASTA scores: opt: 210, E(): 1.4e-06, (25.5% identity in 247 aa overlap). Belongs to the glutaconate CoA-transferase subunit A family. Note that this putative protein may combine with the putative protein encoded by the downstream ORF Rv3552 to form a CoA-transferase that comprises two subunits. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Required for survival in primary murine macrophages, by transposon site hybridization (TraSH) in H37Rv (See Rengarajan et al., 2005). Essential gene for in vitro growth of H37Rv on cholesterol, by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3989896 | 3990774 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3551|Rv3551
VPDKRTALDDAVAQLRSGMTIGIAGWGSRRKPMAFVRAILRSDVTDLTVVTYGGPDLGLLCSAGKVKRVYYGFVSLDSPPFYDPWFAHARTSGAIEAREMDEGMLRCGLQAAAQRLPFLPIRAGLGSSVPQFWAGELQTVTSPYPAPGGGYETLIAMPALRLDAAFAHLNLGDSHGNAAYTGIDPYFDDLFLMAAERRFLSVERIVATEELVKSVPPQALLVNRMMVDAIVEAPGGAHFTTAAPDYGRDEQFQRHYAEAASTQVGWQQFVHTYLSGTEADYQAAVHNFGASR
Bibliography
- Sassetti CM and Rubin EJ [2003]. Genetic requirements for mycobacterial survival during infection. Mutant
- Rengarajan J et al. [2005]. Genome-wide requirements for Mycobacterium tuberculosis adaptation and survival in macrophages. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
