Gene Rv3554
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; C-terminus probably involved in electron transfer in one or several metabolic reactions. |
| Product | Possible electron transfer protein FdxB |
| Comments | Rv3554, (MTCY06G11.01, MTCY03C7.01c), len: 685 aa. Possible fdxB, two-domain protein, with ferredoxin reductase electron transfer component in C-terminal part and unknown function in N-terminal part. Indeed, N-terminal end is similar to O85832 hypothetical 36.1 KDA protein from Sphingomonas aromaticivorans strain F199 (catabolic plasmid pNL1) (309 aa), FASTA scores: opt: 615, E(): 2.5e-30, (33.1% identity in 311 aa overlap); and P73428|SLL1468 hypothetical 36.2 KDA protein from Synechocystis sp. strain PCC 6803 (312 aa), FASTA scores: opt: 317, E(): 4.5e-12, (30.2% identity in 268 aa overlap). And C-terminal end is similar to Q9F9U6|PAAE protein involved in aerobic phenylacetate metabolism from Azoarcus evansii (360 aa), FASTA scores: opt: 935, E(): 7e-50, (43.85% identity in 351 aa overlap); CAC44653|PAAE|SCBAC17A6.08 putative phenylacetic acid degradation NADH oxidoreductase from Streptomyces coelicolor (368 aa), FASTA scores: opt: 93, E(): 9.5e-50, (41.95% identity in 372 aa overlap); Q9FA57|PACI ferredoxin from Azoarcus evansii (360 aa), FASTA scores: opt: 925, E(): 2.9e-49, (43.3% identity in 351 aa overlap); P76081|PAAE_ECOLI|B1392 probable phenylacetic acid degradation NADH oxidoreductase from Escherichia coli strains K12 and W (356 aa), FASTA scores: opt: 910, E(): 2.4e-48, (43.05% identity in 353 aa overlap); Q9APJ6|PAAE electron transfer protein (fragment) from Hyphomicrobium chloromethanicum (241 aa), FASTA scores: opt: 404, E(): 1.7e-17, (35.45% identity in 234 aa overlap); BAB51608|MLL5100 ferredoxin from Rhizobium loti (Mesorhizobium loti) (365 aa), FASTA scores: opt: 316, E(): 5.8e-12, (28.95% identity in 349 aa overlap); etc. C-terminus also similar to P96853|Rv3571|MTCY06G11.18 putative electron transfer protein from Mycobacterium tuberculosis (358 aa), FASTA scores: opt: 450, E(): 3.6e-20, (32.95% identity in 358 aa overlap). Contains PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature. Belongs to the 2FE2S plant-type ferredoxin family. Cofactor: binds a 2FE-2S cluster (by similarity). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3992685 | 3994742 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3554|fdxB
VTDACQAEYAIAAMSTVEMDQAAPESAAHHPLPDPGESVPRLALPTIGIFLATLTAFVGSTTAYISGWIPFWVTIPVNAAVTFVMFTVVHDASHYAISSIRWVNGLFGRLAWLFVGPVVAFPAFGYIHIQHHRHSNDDEQDPDTFASHGSLWVLPLRWSMVEYFYIKYYLPRGRSRPVIEVAETLVMMTLFLTGLIVAIVTGNFWTLAIVFLIPQRIGLTVLAWWFDWLPHHGLEDTQRSNRYRATRNRVGAEWLFTPVLLSQNYHLVHHLHPSVPFYRYLRTWRRNEEAYLERNAAISTVFGQQLNPDEYRQWKELNGRLARLLPVRMPARSSSPHAVLHRIPVASVDPITADATLVTFAVPEALRDAFRFEPGQHVTVRTDLGGQGIRRNYSICAPATRAQLRIAVKHIPGGAFSTFVANELKAGDVLELMTPTGRFGTPLDPLHRKHYVGLVAGSGITPVLSILATTLEIETESRFTLIYGNRTKESTMFRAELDRLESRYADRLEILHVLSSEPLHTPELRGRIDRDKLTRWLTSTLRPAGVDEWFICGPLAMATAVRETLIEHGVDSERIHLELFYGFDTPPATRPSYAGATVTFTLSGQRAIFDLVPGDSILEGALGLRSDAPYACMGGACGTCRAKLIEGNVEMDHNFALRKAELDAGYILTCQSHPTTPFVAVDYDA
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
