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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionFunction unknown, but involved in lipid degradation.
ProductProbable acyl-CoA dehydrogenase FadE32
CommentsRv3563, (MTCY06G11.10), len: 319 aa. Probable fadE32, acyl-CoA dehydrogenase, similar to many e.g. Q9I4V4|PA1020 from Pseudomonas aeruginosa (370 aa), FASTA scores: opt: 347, E(): 7.6e-14, (35.15% identity in 333 aa overlap); Q9RJX3|SCF37.28c from Streptomyces coelicolor (362 aa), FASTA scores: opt: 300, E(): 5.3e-11, (32.4% identity in 349 aa overlap); Q9A5G8|CC2479 from Caulobacter crescentus (344 aa), FASTA scores: opt: 285, E(): 4.1e-10, (30.4% identity in 329 aa overlap); P45857|ACDB_BACSU|MMGC from Bacillus subtilis (379 aa), FASTA scores: opt: 230, E(): 1.1e-07, (25.5% identity in 357 aa overlap); etc. Also similar to other from Mycobacterium tuberculosis eg P96846|FADE33|Rv3564|MTCY06G11.11 (318 aa), FASTA scores: opt: 478, E(): 7.6e-22, (32.9% identity in 292 aa overlap). Could belong to the acyl-CoA dehydrogenases family.
Functional categoryLipid metabolism
ProteomicsIdentified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011).
MutantNon-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Required for survival in primary murine macrophages, by transposon site hybridization (TraSH) in H37Rv (See Rengarajan et al., 2005). Non-essential gene for in vitro growth of H37Rv, but essential for in vitro growth on cholesterol; by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS40042914005250+
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3563|fadE32
VTMEFALNEQQRDFAASIDAALGAADLPGVVRAWAAGDVAPGRKVWQQLANLGVTALGVAEKFDGLGASPVDLVVALERLGRWCVPGPVTESIAVAPILLAHDDQAERSHGLASGELIATVAMPPRVPRAVDADTAGLVLLAGDGSVTEGTPGDCHRSVDPSRRLYEVAASGQAWRAPKDVVARAYEFGALATAAQLVGAGQALLEAAVNYAKQRTQFGRAIGSYQAIKHKLADVHIAIELACPLVYGAAVSLEPRDVSAAKAAASEAALLAARWALQTHGAIGFTCEHDLSLWLLRVQALHSAWGTPQEHRRRVLEAL