Gene Rv3580c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in translation [catalytic activity: ATP + L-cysteine + tRNA(CYS) = AMP + pyrophosphate + L-cysteinyl-tRNA(CYS)]. |
| Product | Cysteinyl-tRNA synthetase 1 CysS1 (cysteine--tRNA ligase 1) (CYSRS 1) (cysteine translase) |
| Comments | Rv3580c, (MTCY06G11.27c), len: 469 aa. Probable cysS1, cysteinyl-tRNA synthetase, equivalent to P57990|SYC1_MYCLE|CYSS1|CYSS|ML0323 cysteinyl-tRNA synthetase 1 from Mycobacterium leprae (473 aa) FASTA scores: opt: 2825, E(): 3.4e-172, (86.5% identity in 467 aa overlap). Also similar to many e.g. Q9L0Q6|SCD8A.08 from Streptomyces coelicolor (613 aa), FASTA scores: opt: 1834, E(): 4.7e-109, (57.5% identity in 461 aa overlap); Q9I2U7|CYSS|PA1795 from Pseudomonas aeruginosa (460 aa) FASTA scores: opt: 1197, E(): 1.2e-68, (41.65% identity in 468 aa overlap); P21888|SYC_ECOLI P21888|CYSS|B0526 from Escherichia coli strain K12 (461 aa), FASTA scores: opt: 1189, E(): 4e-68, (43.0% identity in 463 aa overlap); etc. Belongs to class-I aminoacyl-tRNA synthetase family. Strongly similar to methionyl-tRNA synthetase. |
| Functional category | Information pathways |
| Proteomics | Identified in the cytosol of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4022394 | 4023803 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3580c|cysS1
VTDRARLRLHDTAAGVVRDFVPLRPGHVSIYLCGATVQGLPHIGHVRSGVAFDILRRWLLARGYDVAFIRNVTDIEDKILAKAAAAGRPWWEWAATHERAFTAAYDALDVLPPSAEPRATGHITQMIEMIERLIQAGHAYTGGGDVYFDVLSYPEYGQLSGHKIDDVHQGEGVAAGKRDQRDFTLWKGEKPGEPSWPTPWGRGRPGWHLECSAMARSYLGPEFDIHCGGMDLVFPHHENEIAQSRAAGDGFARYWLHNGWVTMGGEKMSKSLGNVLSMPAMLQRVRPAELRYYLGSAHYRSMLEFSETAMQDAVKAYVGLEDFLHRVRTRVGAVCPGDPTPRFAEALDDDLSVPIALAEIHHVRAEGNRALDAGDHDGALRSASAIRAMMGILGCDPLDQRWESRDETSAALAAVDVLVQAELQNREKAREQRNWALADEIRGRLKRAGIEVTDTADGPQWSLLGGDTK
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
