Gene Rv3585
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in genetic recombination. May play a role in the repair of endogenous alkylation damage. |
| Product | DNA repair protein RadA (DNA repair protein SMS) |
| Comments | Rv3585, (MTV024.03), len: 480 aa. Probable radA, DNA repair protein (see citation below), similar to many e.g. Q9X8L5|SCE94.02 from Streptomyces coelicolor (469 aa), FASTA scores: opt: 1607, E(): 3.1e-84, (56.15% identity in 454 aa overlap); Q9JV51|RADA|NMA0992 from Neisseria meningitidis (serogroup A) (459 aa), FASTA scores: opt: 1275, E(): 2.5e-65, (45.0% identity in 458 aa overlap); and Q9K040|RADA|NMB0782 from Neisseria meningitidis (serogroup B) (459 aa), FASTA scores: opt: 1269, E(): 5.4e-65, (44.5% identity in 456 aa overlap); P37572|RADA_BACSU|SMS from Bacillus subtilis (458 aa), FASTA scores: opt: 1204, E(): 2.7e-61, (39.55% identity in 455 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the RadA family. |
| Functional category | Information pathways |
| Proteomics | Identified in the cytosol of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4026444 | 4027886 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3585|radA
VANARSQYRCSECRHVSAKWVGRCLECGRWGTVDEVAVLSAVGGTRRRSVAPASGAVPISAVDAHRTRPCPTGIDELDRVLGGGIVPGSVTLLAGDPGVGKSTLLLEVAHRWAQSGRRALYVSGEESAGQIRLRADRIGCGTEVEEIYLAAQSDVHTVLDQIETVQPALVIVDSVQTMSTSEADGVTGGVTQVRAVTAALTAAAKANEVALILVGHVTKDGAIAGPRSLEHLVDVVLHFEGDRNGALRMVRGVKNRFGAADEVGCFLLHDNGIDGIVDPSNLFLDQRPTPVAGTAITVTLDGKRPLVGEVQALLATPCGGSPRRAVSGIHQARAAMIAAVLEKHARLAIAVNDIYLSTVGGMRLTEPSADLAVAIALASAYANLPLPTTAVMIGEVGLAGDIRRVNGMARRLSEAARQGFTIALVPPSDDPVPPGMHALRASTIVAALQYMVDIADHRGTTLATPPSHSGTGHVPLGRGT
Bibliography
- Mizrahi V et al. [1998]. DNA repair in Mycobacterium tuberculosis. What have we learnt from the genome sequence? Secondary Function
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
