Gene Rv3589
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in base excision repair. Removes adenine mispaired with 8-OXOG. May repair A.G and A.C mismatches by adenine excision. |
| Product | Probable adenine glycosylase MutY |
| Comments | Rv3589, (MTV024.07), len: 304 aa. Probable mutY, adenine glycosylase (see citation below), equivalent to Q9CBJ0|MUTY|ML1920 probable DNA glycosylase from Mycobacterium leprae (297 aa), FASTA scores: opt: 1592, E(): 2.6e-94, (74.9% identity in 303 aa overlap). Also similar to many DNA glycosylases (generally adenine glycosylases) e.g. Q9S6T7|SCE94.06 from Streptomyces coelicolor (308 aa), FASTA scores: opt: 965, E(): 2.6e-54, (50.5% identity in 297 aa overlap); Q9S6G1|MUTY from Streptomyces antibioticus (307 aa), FASTA scores: opt: 901, E(): 3.1e-50, (48.5% identity in 303 aa overlap); Q9HPQ6|MUTY|VNG1520G from Halobacterium sp. strain NRC-1 (312 aa), FASTA scores: opt: 566, E(): 7.2e-29, (39.85% identity in 296 aa overlap); BAB53965|MLL7523 from Rhizobium loti (Mesorhizobium loti) (396 aa), FASTA scores: opt: 511, E(): 2.8e-25, (39.65% identity in 237 aa overlap); Q05869|MUTY_SALTY|MUTB from Salmonella typhimurium (350 aa), FASTA scores: opt: 421, E(): 3.8e-20, (35.2% identity in 227 aa overlap); etc. Could belong to the nth/MUTY family. |
| Functional category | Information pathways |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4030493 | 4031407 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3589|mutY
MPHILPEPSVTGPRHISDTNLLAWYQRSHRDLPWREPGVSPWQILVSEFMLQQTPAARVLAIWPDWVRRWPTPSATATASTADVLRAWGKLGYPRRAKRLHECATVIARDHNDVVPDDIEILVTLPGVGSYTARAVACFAYRQRVPVVDTNVRRVVARAVHGRADAGAPSVPRDHADVLALLPHRETAPEFSVALMELGATVCTARTPRCGLCPLDWCAWRHAGYPPSDGPPRRGQAYTGTDRQVRGRLLDVLRAAEFPVTRAELDVAWLTDTAQRDRALESLLADALVTRTVDGRFALPGEGF
Bibliography
- Mizrahi V et al. [1998]. DNA repair in Mycobacterium tuberculosis. What have we learnt from the genome sequence? Secondary Function
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
