Gene Rv3610c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Thought to act as an ATP-dependent zinc metallopeptidase, with ATPase and proteolytic activities. Probably has a regulatory role in stress response and specific proteins secretion for adaptation to host environment. |
| Product | Membrane-bound protease FtsH (cell division protein) |
| Comments | Rv3610c, (MT3714, MTCY07H7B.12), len: 760 aa. FtsH, membrane-bound protease (cell division protein) (see citations below), equivalent to Q9CD58|FTSH_MYCLE|ML0222 (alias O69532|FTSH) cell division protein FTSH homolog from Mycobacterium leprae (787 aa), FASTA scores: opt: 4388, E(): 9.6e-205, (87.2% identity in 790 aa overlap). Also highly similar to many FTSH proteins e.g. O52395|FTSH from Mycobacterium smegmatis (769 aa), FASTA scores: opt: 3976, E(): 7.6e-185, (82.4% identity in 761 aa overlap); Q9X8I4|SCE9.11c from Streptomyces coelicolor (668 aa), FASTA scores: opt: 2417, E(): 1.4e-109, (57.2% identity in 668 aa overlap); P72991|FTH4_SYNY3|SLR1604 from Synechocystis sp. strain PCC 6803 (616 aa), FASTA scores: opt: 1926, E(): 7.2e-86, (49.35% identity in 612 aa overlap); P28691|FTSH_ECOLI|HFLB|MRSC|TOLZ|B3178 from Escherichia coli strain K12 (644 aa), FASTA scores: opt: 1859, E(): 1.3e-82, (48.95% identity in 605 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00674 AAA-protein family signature. Belongs to the AAA family of ATPases and peptidase family M41 (zinc metalloprotease). Cofactor: binds one zinc ion (potential). Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS; predicted transmembrane protein (See Gu et al., 2003). Identified in the cytosol and cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al.,2003). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4050601 | 4052883 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3610c|ftsH
MNRKNVTRTITAIAVVVLLGWSFFYFSDDTRGYKPVDTSVAITQINGDNVKSAQIDDREQQLRLILKKGNNETDGSEKVITKYPTGYAVDLFNALSAKNAKVSTVVNQGSILGELLVYVLPLLLLVGLFVMFSRMQGGARMGFGFGKSRAKQLSKDMPKTTFADVAGVDEAVEELYEIKDFLQNPSRYQALGAKIPKGVLLYGPPGTGKTLLARAVAGEAGVPFFTISGSDFVEMFVGVGASRVRDLFEQAKQNSPCIIFVDEIDAVGRQRGAGLGGGHDEREQTLNQLLVEMDGFGDRAGVILIAATNRPDILDPALLRPGRFDRQIPVSNPDLAGRRAVLRVHSKGKPMAADADLDGLAKRTVGMTGADLANVINEAALLTARENGTVITGPALEEAVDRVIGGPRRKGRIISEQEKKITAYHEGGHTLAAWAMPDIEPIYKVTILARGRTGGHAVAVPEEDKGLRTRSEMIAQLVFAMGGRAAEELVFREPTTGAVSDIEQATKIARSMVTEFGMSSKLGAVKYGSEHGDPFLGRTMGTQPDYSHEVAREIDEEVRKLIEAAHTEAWEILTEYRDVLDTLAGELLEKETLHRPELESIFADVEKRPRLTMFDDFGGRIPSDKPPIKTPGELAIERGEPWPQPVPEPAFKAAIAQATQAAEAARSDAGQTGHGANGSPAGTHRSGDRQYGSTQPDYGAPAGWHAPGWPPRSSHRPSYSGEPAPTYPGQPYPTGQADPGSDESSAEQDDEVSRTKPAHG
Bibliography
- Amara RR et al. [1998]. Characterization of novel immunodominant antigens of Mycobacterium tuberculosis. Product Localization
- Anilkumar G et al. [1998]. Cloning and expression of the gene coding for FtsH protease from Mycobacterium tuberculosis H37Rv. Sequence Product Localization
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Ribeiro-Guimarães ML et al. [2007]. Comparative genomics of mycobacterial proteases. Homology
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
