Gene Rv3625c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Thought to be involved in a cell cycle process. |
| Product | Possible cell cycle protein MesJ |
| Comments | Rv3625c, (MT3727, MTCY15C10.27), len: 323 aa. Possible mesJ, cell cycle protein, equivalent to O69538|Y0C5_MYCLE|ML0213|MLCB2548.18c hypothetical 34.1 KDA protein from Mycobacterium leprae (323 aa) FASTA scores: opt: 1592, E(): 9e-92, (78.0% identity in 327 aa overlap). Similar to bacterial hypothetical proteins Q9X8I6|SCE9.13c from Streptomyces coelicolor (352 aa) FASTA scores: opt: 705, E(): 1.4e-36, (47.85% identity in 305 aa overlap); and Q9HXZ3|PA3638 from Pseudomonas aeruginosa (442 aa), FASTA scores: opt: 382, E(): 2e-16, (40.6% identity in 271 aa overlap). But also similar (or with similarity) to bacterial cell cycle proteins (MESJ) e.g. Q9KPX0|VC2242 MESJ protein from Vibrio cholerae (440 aa), FASTA scores: opt: 363, E(): 3e-15, (34.8% identity in 253 aa overlap); Q9RV23|DR1207 (600 aa) cell cycle protein MESJ (putative/cytosine deaminase-related protein) from Deinococcus radiodurans (600 aa), FASTA scores: opt: 310, E(): 7.6e-12, (36.6% identity in 265 aa overlap) (similar only at the N-terminal end); Q9PFJ8|XF0659 cell cycle protein from Xylella fastidiosa (437 aa), FASTA scores: opt: 301, E(): 2.1e-11, (35.05% identity in 271 aa overlap); P52097|MESJ_ECOLI|B0188 putative cell cycle protein MESJ from Escherichia coli strain K12(432 aa) FASTA scores: opt: 299, E(): 2.8e-11, (34.65% identity in 277 aa overlap); etc. Belongs to the UPF0072 (MESJ/YCF62) family. |
| Functional category | Cell wall and cell processes |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4063901 | 4064872 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3625c|mesJ
MDRQSAVAQLRAAAEQFARVHLDACDRWSVGLSGGPDSLALTAVAARLWPTTALIVDHGLQPGSATVAETARIQAISLGCVDARVLCVQVGAAGGREAAARSARYSALEEHRDGPVLLAHTLDDQAETVLLGLGRGSGARSIAGMRPYDPPWCRPLLGVRRSVTHAACRELGLTAWQDPHNTDRRFTRTRLRTEVLPLLEDVLGGGVAEALARTATALREDTDLIDTIAAQALPGAAVAGSRGQELSTSALTALPDAVRRRVIRGWLLAGGATGLTDRQIRGVDRLVTAWRGQGGVAVGSTLRGQRLVAGRRDGVLVLRREPV
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
