Gene Rv3633
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved protein |
| Comments | Rv3633, (MTCY15C10.19c), len: 291 aa. Conserved protein, similar to Q9X5S6|MMCH from Streptomyces lavendulae (254 aa), FASTA scores: opt: 368, E(): 3.2e-16, (35.05% identity in 194 aa overlap); Q9APW1 hypothetical 32.7 KDA protein from Pseudomonas aeruginosa (295 aa), FASTA scores: opt: 359, E(): 1.3e-15, (37.65% identity in 170 aa overlap); Q9APV4 hypothetical 34.1 KDA protein from Pseudomonas aeruginosa (309 aa), FASTA scores: opt: 316, E(): 7.6e-13, (28.65% identity in 262 aa overlap). And some similarity to Q9HGD7|FUM9 FUM9P from Gibberella moniliformis (300 aa), FASTA scores: opt: 254, E(): 6.5e-09, (29.95% identity in 157 aa overlap); and P47181|YJ9S_YEAST|YJR154W|J2240 hypothetical 39.0 KDA protein from Saccharomyces cerevisiae (Baker's yeast) (346 aa), FASTA scores: opt: 190, E(): 8.5e-05, (26.75% identity in 127 aa overlap). Also similar to P71782|YF01_MYCTU|Rv1501|MT1550|MTCY277.23 from Mycobacterium tuberculosis (273 aa), FASTA scores: opt: 286, E(): 5.5e-11, (27.5% identity in 280 aa overlap). |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Transcriptomics | mRNA identified by microarray analysis and up-regulated after 4h, 24h and 96h of starvation (see citation below). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4071791 | 4072666 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3633|Rv3633
MTQSSSVERLVGEIDEFGYTVVEDVLDADSVAAYLADTRRLERELPTVIANSTTVVKGLARPGHVPVDRVDHDWVRIDNLLLHGTRYEALPVHPKLLPVIEGVLGRDCLLSWCMTSNQLPGAVAQRLHCDDEMYPLPRPHQPLLCNALIALCDFTADNGATQVVPGSHRWPERPSPPYPEGKPVEINAGDALIWNGSLWHTAAANRTDAPRPALTINFCVGFVRQQVNQQLSIPRELVRCFEPRLQELIGYGLYAGKMGRIDWRPPADYLDADRHPFLDAVADRLQTSVRL
Bibliography
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Mazandu GK et al. [2012]. Function prediction and analysis of mycobacterium tuberculosis hypothetical proteins. Function
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
