Gene Rv3644c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | DNA polymerase is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. |
| Product | Possible DNA polymerase |
| Comments | Rv3644c, (MTCY15C10.08), len: 401 aa. Possible DNA polymerase, equivalent to O69546|MLCB2548.29c|ML0202 hypothetical 42.7 KDA protein from Mycobacterium leprae (405 aa), FASTA scores: opt: 2180, E(): 6.1e-116, (84.4% identity in 404 aa overlap). Similar (in totality or in first 200 aa) to DNA polymerases III, delta' or gamma subunit, e.g. Q9X906|SCH5.03c putative DNA polymerase from Streptomyces coelicolor (401 aa), FASTA scores: opt: 1022, E(): 1.5e-50, (47.05% identity in 404 aa overlap); Q9RRS5|DR2410 DNA polymerase III, tau/gamma subunit from Deinococcus radiodurans (615 aa), FASTA scores: opt: 370, E(): 1.3e-13, (29.95% identity in 394 aa overlap); P28631|HOLB_ECOLI|B1099 DNA polymerase III, delta' subunit from Escherichia coli strain K12 (334 aa), FASTA scores: opt: 345, E(): 2.2e-12, (33.45% identity in 239 aa overlap); Q9JTS1|DNAZX|NMA1656 DNA polymerase III tau and gamma chains from Neisseria meningitidis (serogroup A) (709 aa), FASTA scores: opt: 346, E(): 3.3e-12, (28.55% identity in 364 aa overlap); etc. |
| Functional category | Information pathways |
| Proteomics | Identified in the cytosol of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4081516 | 4082721 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3644c|Rv3644c
MSGVFTRLVGQQAVEAELLATAKAARRDSAHSAGGGGTMTHAWLLTGPPGSGRSVAALCFAAALQCTSGGEPGCGRCRACTTTLAGTHADVRRVIPEGLSIGVDEMRAIVQIAARRPTTGHWQIVVIEDADRLTEGAANALLKVVEEPPPSTVFLLCAPSVDPEDIAVTLRSRCRHVALVTPSTHAIAQVLSDGDGLDPDTANWAASVSGGHVGRARRLATDPQARQRRERALGLARDAATPSRAYAAAEELVAGAEAEALALTAQRIEAETEELRTALGAGGTGKGTGAALRGATGAMKDLERRQKSRQTRASRDALDRALIDLATYFRDALLVAAHAGGVRANHPDMADRVAALAAHAPPERLLRCIEAVLACREALAVNVKPKFAVDAMVATIGQELR
Bibliography
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
