Gene Rv3711c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease [catalytic activity: N deoxynucleoside triphosphate = N diphosphate + {DNA}(N)]. |
| Product | Probable DNA polymerase III (epsilon subunit) DnaQ |
| Comments | Rv3711c, (MTV025.059c), len: 329 aa. Probable dnaQ, DNA polymerase III, epsilon subunit, similar to many e.g. Q9RJ41|SCI8.12 from Streptomyces coelicolor (328 aa), FASTA scores: opt: 509, E(): 4.2e-25, (41.6% identity in 315 aa overlap); Q9JYS6|NMB1451 from Neisseria meningitidis (serogroup B) (and Q9JTR5|MA1665 from serogroup A) (470 aa), FASTA scores: opt: 247, E(): 2.6e-08, (33.15% identity in 172 aa overlap); O83649|DP3E_TREPA|DNAQ|TP0643 from Treponema pallidum (215 aa), FASTA scores: opt: 240, E(): 3.7e-08, (29.65% identity in 162 aa overlap); P03007|DP3E_ECOLI|MUTD|B0215 from Escherichia coli strain K12 (243 aa), FASTA scores: opt: 208, E(): 4.5e-06, (28.4% identity in 169 aa overlap); etc. Also similar to Q10384|YL91_MYCTU|Rv2191|MTCY190.02 from Mycobacterium tuberculosis (645 aa), FASTA scores: opt: 260, E(): 5e-09, (28.55% identity in 301 aa overlap). |
| Functional category | Information pathways |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4155740 | 4156729 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3711c|dnaQ
VSHTWGRPASHQDRGWAVIDVETSGFRPGQARIISLAVLGLDAAGRLEQSVVSLLNPKVDPGPTHVHGLTAAMLDGQPQFADIAGEVVDVLRGRTLVAHNVAFDYAFLAAEAEIAEAELPVDFVMCTVELARRLQLGVDNLRLETLAAHWGVPQQRPHDAFDDVRVLTGILAAALESARELDVWLPVHPVTRRRWPNGRVTHDELRPLKAVAARMACPYLNPGRYVQGRPLVQGMRVGLAAEVKRTHEELVERILHAGLAYSDVVDRDTSLVVCNATAPEHGKGYHALQLGVPVMPEARFMECIGAVVGGASVEDFTDVAPVEKQLALF
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
