Gene Rv3727
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; probably involved in cellular metabolism. |
| Product | Possible oxidoreductase |
| Comments | Rv3727, (MTV025.075), len: 602 aa. Possible oxidoreductase, similar to several plants phytoene dehydrogenases/desaturases e.g. Q9HSE1|CRTI3|VNG0277G phytoene dehydrogenase from Halobacterium sp. strain NRC-1 (541 aa), FASTA scores: opt: 299, E(): 1.1e-10, (29.85% identity in 576 aa overlap); Q9FZL6|CITPDS1 phytoene desaturase from Citrus unshiu (Satsuma orange) (553 aa), FASTA scores: opt: 164, E(): 0.018, (24.2% identity in 434 aa overlap); Q07356|CRTI_ARATH|PDS|AT4G14210|DL3145c phytoene dehydrogenase precursor from Arabidopsis thaliana (Mouse-ear cress) (566 aa), FASTA scores: opt: 163, E(): 0.021, (23.95% identity in 434 aa overlap); etc. N-terminal end similar to O69871|SC1C3.29 putative protoporphyrinogen oxidase (fragment) from Streptomyces coelicolor (61 aa), FASTA scores: opt: 154, E(): 0.012, (60.45% identity in 43 aa overlap). The region between aa 155-310 is highly similar to Q49778|B2126_C1_169 from Mycobacterium leprae (159 aa), FASTA scores: opt: 437, E(): 1.5e-19, (46.6% identity in 161 aa overlap). And the region between aa 462-546 is highly similar to the N-terminal end of Q50003|U1764T from Mycobacterium leprae (155 aa), FASTA scores: opt: 277, E(): 8.3e-10, (57.65% identity in 85 aa overlap). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4172955 | 4174763 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3727|Rv3727
MKPSPADTHVVIAGAGIAGLAAAMILAEAGVRVTLCEAASEAGGKAKSLRLADGHPTEHSLRVYTDTYQTLLTLFSRIPTEHDRTVLDNLVGVSMVSATAQGVIGRIAAPVALQRRRPTFARIIGKVVEPPRQLVRILLRGPMVIVGLAQRGVPATDVLHYLYAHLRLLWMCRERLLAELGDISYADYLQLGCKSAQAQEFFSAVPRIYVAARTSAEAAAIAPIVLKGLFRLKSNCPSALNDAKLPAIMMMDGPTSERMVDPWIRHLTRLGVDIHFNTRVGDLEFDDGRVTALISSDGRRFACDYALLAVPYLTLRELAKSAHVKRYLPQLTQQHALALEASNGIQCFLRDLPATWPPFIRPGVVTTHLQSQWSLVCVLQGEGFWKNVRLPEGTRYVLSITWSDVETPGPVFDRPLSECTPDEILTECLTQCGLDKSNVLGWRIDHELKHLDEAEYEKVASELPPHLVSAPARGQRMVNFSPLTVLMPGARHRSPGICTSVPNLLLAGEVIYSPDLTLFVPTMEKAACSGYLAARQIMNMVASHAAPLRIDFRDPAPFAVLRRVDRWFWSRRRRPPDRSTFATPPTAMPAPSHLTDVDRSAS
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
