Gene Rv3764c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Sensor part of a two component regulatory system. |
| Product | Possible two component sensor kinase TcrY |
| Comments | Rv3764c, (MTV025.112c), len: 475 aa. Possible tcrY, histidine protein kinase, part of a two-component regulatory system, similar to others e.g. Q9ADN6|2SC10A7.25 putative two component system histidine kinase from Streptomyces coelicolor (524 aa), FASTA scores: opt: 1332, E(): 5.4e-70, (49.9% identity in 477 aa overlap); Q9L3C1|KB|CAC42479 putative histidine kinase from Amycolatopsis mediterranei (469 aa), FASTA scores: opt: 515, E(): 1.4e-22, (36.1% identity in 313 aa overlap); P72560 histidine protein kinase from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (438 aa), FASTA scores: opt: 480, E(): 1.4e-20, (40.1% identity in 232 aa overlap); P30847|P76401|BAES_ECOLI|B2078 sensor protein from Escherichia coli strain K12 (467 aa); etc. Also similar to others from Mycobacterium tuberculosis e.g. P96368|Rv1032c|MTCY10G2.17 (509 aa), FASTA scores: opt: 1007, E(): 4e-51, (43.5% identity in 416 aa overlap); and P71815|Rv0758|MTCY369.03 (485 aa), FASTA scores: opt: 738, E(): 1.6e-35, (28.6% identity in 438 aa overlap). Equivalent to AAK48235 from Mycobacterium tuberculosis strain CDC1551 (506 aa) but shorter 31 aa. |
| Functional category | Regulatory proteins |
| Proteomics | Identified in the cytosol and cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Found to be deleted (partially or completely) in one or more clinical isolates (See Tsolaki et al., 2004). M. tuberculosis H37Rv tcrXY mutant shows increased virulence in SCID mice (See Parish et al., 2004). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4209582 | 4211009 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3764c|tcrY
VGITAATEMALRRHLVAQLDNQLGGTSYRSVLMYPEKMPRPPWRHETHNYIRSGPGPRFLDAPGQPAGMVAAVVSDGTTVAAGYLTGSGSRAALTSTGRSQLERIAGSRTPLTLDLDGLGRYRVLAAPSRNGHDVIVTGLSMGNVDATMLQMLIIFGIVTVIALVAATTAGIVIIKRALAPLRRVAQTASEVVDLPLDRGEVKLPVRVPEPDANPSTEVGQLGSALNRMLDHIAAALSARQASETCVRQFVADASHELRTPLAAIRGYTELTQRIGDDPEAVAHAMSRVASETERITRLVEDLLLLARLDSGRPLERGPVDMSRLAVDAVSDAHVAGPDHQWALDLPPEPVVIPGDAARLHQVVTNLLANARVHTGPGTIVTTRLSTGPTHVVLQVIDNGPGIPAALQSEVFERFARGDTSRSRQAGSTGLGLAIVSAVVKAHNGTITVSSSPGYTEFAVRLPLDGWQPLESSPR
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Parish T et al. [2003]. Deletion of two-component regulatory systems increases the virulence of Mycobacterium tuberculosis. Mutant Secondary Function
- Tsolaki AG, Hirsh AE, DeRiemer K, Enciso JA, Wong MZ, Hannan M, Goguet de la Salmoniere YO, Aman K, Kato-Maeda M and Small PM [2004]. Functional and evolutionary genomics of Mycobacterium tuberculosis: insights from genomic deletions in 100 strains. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
