Gene Rv3767c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Possible methyltransferase |
| Product | Possible S-adenosylmethionine-dependent methyltransferase |
| Comments | Rv3767c, (MTV025.115c, MTCY13D12.01), len: 314 aa. Possible S-adenosylmethionine-dependent methyltransferase (see Grana et al., 2007), similar to other Mycobacterium tuberculosis hypothetical proteins e.g. P96823|Rv0146|MTCI5.20 34.0 KDA protein (310 aa), FASTA scores: opt: 909, E(): 5.3e-50, (48.1% identity in 316 aa overlap); O53686|Rv0281|MTV035.09 (302 aa), FASTA scores: opt: 802, E(): 2.8e-43, (45.2% identity in 314 aa overlap); Q50726|YX99_MYCTU|Rv3399|MT3507|MTCY78.29c (348 aa), FASTA scores: opt: 796, E(): 7.6e-43, (45.35% identity in 302 aa overlap); MTCY78_30; MTCY31_23; MTCY210_45; MTCY4C12_14; MTY13D12_21, MTCI5_19; MTCY180_22; etc. Contains probable N-terminal signal sequence |
| Functional category | Lipid metabolism |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Transcriptomics | DNA microarrays show higher level of expression in M. tuberculosis H37Rv than in phoP|Rv0757 mutant (See Walters et al., 2006). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Found to be deleted (partially or completely) in one or more clinical isolates (See Tsolaki et al., 2004). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4212996 | 4213940 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3767c|Rv3767c
VPRTDNDSWAITESVGATALGVAAARAAETESDNPLINDPFARIFVDAAGDGIWSMYTNRTLLAGATDLDPDLRAPIQQMIDFMAARTAFFDEYFLATADAGVRQVVILASGLDSRAWRLPWPDGTVVYELDQPKVLEFKSATLRQHGAQPASQLVNVPIDLRQDWPKALQKAGFDPSKPCAWLAEGLVRYLPARAQDLLFERIDALSRPGSWLASNVPGAGFLDPERMRRQRADMRRMRAAAAKLVETEISDVDDLWYAEQRTAVAEWLRERGWDVSTATLPELLARYGRSIPHSGEDSIPPNLFVSAQRATS
Bibliography
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Tsolaki AG, Hirsh AE, DeRiemer K, Enciso JA, Wong MZ, Hannan M, Goguet de la Salmoniere YO, Aman K, Kato-Maeda M and Small PM [2004]. Functional and evolutionary genomics of Mycobacterium tuberculosis: insights from genomic deletions in 100 strains. Mutant
- Walters SB et al. [2006]. The Mycobacterium tuberculosis PhoPR two-component system regulates genes essential for virulence and complex lipid biosynthesis. Transcriptome
- Graña M et al. [2007]. The crystal structure of M. leprae ML2640c defines a large family of putative S-adenosylmethionine-dependent methyltransferases in mycobacteria. Function
- Fontán PA et al. [2008]. Mycobacterium tuberculosis sigma factor E regulon modulates the host inflammatory response. Regulon
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
