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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionCould possibly oxidize fatty acids using specific components [catalytic activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O].
ProductPossible enoyl-CoA hydratase EchA21 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase)
CommentsRv3774, (MTCY13D12.08), len: 274 aa. Possible echA21, enoyl-CoA hydratase, equivalent to Q9CD94|ECHA1|ML0120 putative enoyl-CoA hydratase from Mycobacterium leprae (278 aa), FASTA scores: opt: 1593, E(): 2.2e-92, (88.3% identity in 274 aa overlap). Also similar to others e.g. Q9I2S4|PA1821 from Pseudomonas aeruginosa (270 aa), FASTA scores: opt: 761, E(): 2e-40, (42.3% identity in 267 aa overlap); Q9FHR8 from Arabidopsis thaliana (Mouse-ear cress) (278 aa) FASTA scores: opt: 638, E(): 9.9e-33, (39.4% identity in 269 aa overlap); Q9AB78|CC0353 from Caulobacter crescentus (286 aa), FASTA scores: opt: 601, E(): 2.1e-31, (39.25% identity in 266 aa overlap); etc.
Functional categoryLipid metabolism
ProteomicsIdentified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). Translational start site supported by proteomics data (See de Souza et al., 2011) (See Kelkar et al., 2011).
MutantNon-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS42188494219673+
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3774|echA21
MGETYESVTVETKDQVAQVTLIGPGKGNAMGPAFWSEMPEVFHALDADREVRAIVITGSGKNFSYGLDVPAMGGMFAPLIADGALARPRTDFHTEILRMQKAINAVADCRTPTIAAVQGWCIGGAVDLISAVDIRYASADAKFSVREVKLAIVADMGSLARLPLILSDGHLRELALTGKNIDAARAEKIGLVNDVYDDADQTLAAAHATAAEIAANPPLAVYGIKDVLDQQRTSAVSENLRYVAAWNAAFLPSKDLTEGISATFAKRPPQFTGE