Gene Rv3775
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; lipolytic enzyme probably involved in cellular metabolism. |
| Product | Probable lipase LipE |
| Comments | Rv3775, (MTCY13D12.09), len: 415 aa. Probable lipE, hydrolase lipase, equivalent to Q9CD95|LIPE|ML0119 probable hydrolase from Mycobacterium leprae (411 aa), FASTA scores: opt: 2418, E(): 6.4e-144, (84.75% identity in 406 aa overlap). Also similar to other esterases e.g. Q9ABH2|CC0255 esterase a from Caulobacter crescentus (374 aa), FASTA scores: opt: 427, E(): 2.4e-19, (28.9% identity in 391 aa overlap); O87861|ESTA esterase a from Streptomyces chrysomallus (389 aa), FASTA scores: opt: 417, E(): 1e-18, (31.0% identity in 361 aa overlap); Q9RK50|SCF12.08 putative esterase from Streptomyces coelicolor (376 aa), FASTA scores: opt: 385, E(): 1e-16, (31.35% identity in 373 aa overlap); etc. Also similar to proteins from Mycobacterium tuberculosis e.g. P71778|Rv1497|MTCY277.19 hypothetical 45.8 KDA protein (429 aa), FASTA scores: opt: 457, E(): 3.5e-21, (30.4% identity in 395 aa overlap). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4219685 | 4220932 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3775|lipE
MRAGDGKIRVPADLDAVTATGEEDHSEIDGAAVDRIWRAARHWYRAGMHPAIQLCIRHHGRVVLNRAIGHGWGNAPTDEADAEKIPVTTDTPFCVYSAAKAITATVVHMLVERGHFALDDRVCEYLPSYTSHGKHRTTIRHVLTHSAGVPFPTGPRPDVRRADDHEYAVERLGELRPLYRPGLVHIYHALTWGPLMREIVYAATGKEIREILATEILDPLGFRWTNFGVAERDVPLVAPSHATGRQLPPVIAAVFRKAIGGTVHEIIPYTNTPFFLSTILPSSNTVSTANELSRFMEILRRGGELDGVRVLSPETLRGAVTECRRLRPDFATGLMPLRWGTGFMLGSAKYGPFGRNAPAAFGHLGLVNIAVWADPERALSGGLISSGKPGRDPEAGRYGALLNAITAEIPRASSG
Bibliography
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Deb C, Daniel J, Sirakova TD, Abomoelak B, Dubey VS and Kolattukudy PE [2006]. A novel lipase belonging to the hormone-sensitive lipase family induced under starvation to utilize stored triacylglycerol in Mycobacterium tuberculosis. Product Transcriptome
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
