Gene Rv3807c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown |
| Product | Possible conserved transmembrane protein |
| Comments | Rv3807c, (MTV026.12), len: 165 aa. Possible conserved transmembrane protein, equivalent to Q9CDB6|ML0094 putative membrane protein from Mycobacterium leprae (192 aa), FASTA scores: opt: 714, E(): 2.4e-38, (72.85% identity in 151 aa overlap). Also highly similar to Q9KZA3|SC5G8.11 putative integral membrane protein from Streptomyces coelicolor (169 aa), FASTA scores: opt: 324, E(): 1.1e-13, (41.5% identity in 159 aa overlap); and similar in part to others e.g. Q9K3L3|SCG20A.27 putative integral membrane protein from Streptomyces coelicolor (230 aa), FASTA scores: opt: 277, E(): 1.3e-10, (41.65% identity in 168 aa overlap); P72269|ORF8 hypothetical protein from Rhodococcus erythropolis (487 aa) FASTA scores: opt: 229, E(): 2.7e-07, (36.25% identity in 149 aa overlap); O86625|SC3A7.24c putative integral membrane protein from Streptomyces coelicolor (201 aa) FASTA scores: opt: 200, E(): 9.1e-06, (34.95% identity in 146 aa overlap); Q9KYD7|SCD72A.19 putative integral membrane protein from Streptomyces coelicolor (238 aa) FASTA scores: opt: 178, E(): 0.00026, (35.7% identity in 112 aa overlap); etc. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004). |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Required for survival in primary murine macrophages, by transposon site hybridization (TraSH) in H37Rv (See Rengarajan et al., 2005). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4269840 | 4270337 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3807c|Rv3807c
MVAVQSALVDRPGMLATARGLSHFGEHCIGWLILALLGAIALPRRRREWLVAGAGAFVAHAIAVLIKRLVRRQRPDHPAIAVNVDTPSQLSFPSAHATSTTAAALLMGRATGLPLPVVLVPPMALSRILLGVHYPSDVAVGVALGATVGAIVDSVGGGRQRARKR
Bibliography
- Marmiesse M, Brodin P, Buchrieser C, Gutierrez C, Simoes N, Vincent V, Glaser P, Cole ST and Brosch R [2004]. Macro-array and bioinformatic analyses reveal mycobacterial 'core' genes, variation in the ESAT-6 gene family and new phylogenetic markers for the Mycobacterium tuberculosis complex. Homology
- Rengarajan J et al. [2005]. Genome-wide requirements for Mycobacterium tuberculosis adaptation and survival in macrophages. Mutant
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
