Gene Rv3816c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; probably involved in cellular metabolism. |
| Product | Possible acyltransferase |
| Comments | Rv3816c, (MTCY409.14), len: 259 aa. Possible acyltransferase, equivalent to Q9CDC0|ML0087 putative acyltransferase from Mycobacterium leprae (257 aa) FASTA scores: opt: 1401, E(): 1.5e-80, (81.9% identity in 254 aa overlap). Also highly similar to many putative acyltransferases and hypothetical proteins e.g. Q9K3R3|2SCG4.01 putative acyltransferase from Streptomyces coelicolor (242 aa), FASTA scores: opt: 758, E(): 2.4e-40, (51.7% identity in 234 aa overlap); Q9ZBS1|SC7A1.02 putative acyltransferase from Streptomyces coelicolor (264 aa), FASTA scores: opt: 312, E(): 2e-12, (29.55% identity in 237 aa overlap); O67841|AAS|AQ_2058 2-acylglycerophosphoethanolamine acyltransferase from Aquifex aeolicus (211 aa), FASTA scores: opt: 281, E(): 1.5e-10, (32.7% identity in 162 aa overlap); etc. Also highly similar to upstream ORFs O07808|Rv3815c|MTCY409.15 putative acyltransferase from Mycobacterium tuberculosis (251 aa), FASTA scores: opt: 847, E(): 6.7e-46, (55.7% identity in 246 aa overlap); and O07809|Rv3814c|MTCY409.16 putative acyltransferase from Mycobacterium tuberculosis (261 aa), FASTA scores: opt: 776, E(): 1.9e-41, (50.9% identity in 228 aa overlap). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the detergent phase of Triton X-114 extracts of M. tuberculosis H37Rv membranes using CEGE and MALDI-TOF-MS (See Sinha et al., 2005). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4280792 | 4281571 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3816c|Rv3816c
VEPVYGTVIRLARLSWRIQGLKITVTGVDNLPTSGGAVVAINHTSYLDFTFAGLPAYQQGLGRKVRFMAKQEVFDHKITGPIMRSLRHIPVDRQDGSASYDAAVRMLKAGELVGVYPEATISRSFEIKEFKTGAARMAIEAGVPIVPHIVWGAQRIWTKDRPKKLFRPKVPVTIVVGERIEPTLPTAELNGLLHSRMQHLLERAQELYGPHPAGEFWVPHRLGGGAPSLAEAARLDAQEAAVRAARRAQRAHPAGAPEQ
Bibliography
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sinha S, Kosalai K, Arora S, Namane A, Sharma P, Gaikwad AN, Brodin P and Cole ST [2005]. Immunogenic membrane-associated proteins of Mycobacterium tuberculosis revealed by proteomics. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
