Gene Rv3826
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown, but involved in lipid degradation. |
| Product | Probable fatty-acid-AMP ligase FadD23 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) |
| Comments | Rv3826, (MTCY409.04c), len: 584 aa. Probable fadD23, fatty-acid-AMP synthetase, highly similar to P71495 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA scores: opt: 2571, E(): 4.4e-146, (66.15% identity in 576 aa overlap); Q9CD79|FADD28|ML0138 acyl-CoA synthetase from Mycobacterium leprae (579 aa) FASTA scores: opt: 2520, E(): 4.9e-143, (65.2% identity in 575 aa overlap); P54200|FD21_MYCLE putative fatty-acid--CoA ligase (acyl-CoA synthetase) from Mycobacterium leprae (579 aa), FASTA scores: opt: 2330, E(): 1.1e-131, (60.2% identity in 578 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. P96290|FADD28|Rv2941|MTCY24G1.08c (580 aa), FASTA scores: opt: 2587, E(): 4.9e-147, (66.5% identity in 576 aa overlap); O53903|FADD24|Rv1529|MTV045.03 (584 aa), FASTA scores: opt: 2457, E(): 2.9e-139, (63.35% identity in 584 aa overlap); Q50586|FADD25|Rv1521|MT1572|MTCY19G5.07 (583 aa) FASTA scores: opt: 2389, E(): 3.3e-135, (61.45% identity in 581 aa overlap); etc. |
| Functional category | Lipid metabolism |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). M. tuberculosis Erdman fadD23|Rv3826 transposon mutant does not produce sulfolipid (See Lynett and Stokes, 2007). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4299812 | 4301566 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3826|fadD23
MVSLSIPSMLRQCVNLHPDGTAFTYIDYERDSEGISESLTWSQVYRRTLNVAAEVRRHAAIGDRAVILAPQGLDYIVAFLGALQAGLIAVPLSAPLGGASDERVDAVVRDAKPNVVLTTSAIMGDVVPRVTPPPGIASPPTVAVDQLDLDSPIRSNIVDDSLQTTAYLQYTSGSTRTPAGVMITYKNILANFQQMISAYFADTGAVPPLDLFIMSWLPFYHDMGLVLGVCAPIIVGCGAVLTSPVAFLQRPARWLQLMAREGQAFSAAPNFAFELTAAKAIDDDLAGLDLGRIKTILCGSERVHPATLKRFVDRFSRFNLREFAIRPAYGLAEATVYVATSQAGQPPEIRYFEPHELSAGQAKPCATGAGTALVSYPLPQSPIVRIVDPNTNTECPPGTIGEIWVHGDNVAGGYWEKPDETERTFGGALVAPSAGTPVGPWLRTGDSGFVSEDKFFIIGRIKDLLIVYGRNHSPDDIEATIQEITRGRCAAIAVPSNGVEKLVAIVELNNRGNLDTERLSFVTREVTSAISTSHGLSVSDLVLVAPGSIPITTSGKVRRAECVKLYRHNEFTRLDAKPLQASDL
Bibliography
- Trivedi OA et al. [2004]. Enzymic activation and transfer of fatty acids as acyl-adenylates in mycobacteria. Function Product
- Lynett J et al. [2007]. Selection of transposon mutants of Mycobacterium tuberculosis with increased macrophage infectivity identifies fadD23 to be involved in sulfolipid production and association with macrophages. Mutant
- Fontán PA et al. [2008]. Mycobacterium tuberculosis sigma factor E regulon modulates the host inflammatory response. Regulon
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
