Gene Rv3827c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Required for the transposition of the insertion sequence IS1537. |
| Product | Possible transposase |
| Comments | Rv3827c, (MTCY409.03), len: 408 aa. Possible transposase within IS1537 element, similar to several transposases e.g. O83029|TNPC|DR2324|DR0666|DR0978|DR1381|DR1651|DR1933 transposase from Deinococcus radiodurans(408 aa) FASTA scores: opt: 302, E(): 3.9e-12, (30.75% identity in 358 aa overlap); Q9RXX7|DR0178 putative transposase from Deinococcus radiodurans (409 aa), FASTA scores: opt: 297, E(): 8.2e-12, (31.1% identity in 360 aa overlap); P73816|SLR2062 transposase from Synechocystis sp. strain PCC 6803 (400 aa), FASTA scores: opt: 296, E(): 9.3e-12, (30.05% identity in 353 aa overlap); etc. Highly similar to proteins from Mycobacterium tuberculosis e.g. O33333|Rv2791c|MTV002.56c transposase (459 aa) FASTA scores: opt: 2211, E(): 9.4e-136, (87.75% identity in 367 aa overlap); P95117|Rv2978c|MTCY349.09 hypothetical 51.4 KDA protein (459 aa), FASTA scores: opt: 2165, E(): 9e-133, (85.85% identity in 367 aa overlap); Q10809|YS85_MYCTU|Rv2885c|MT2953|MTCY274.16c hypothetical 51.3 KDA protein (460 aa), FASTA scores: opt: 2127, E(): 2.6e-130, (83.95% identity in 368 aa overlap); O0777|Rv0606|MTCY19H5.16c probable transposase (fragment) (247 aa), FASTA scores: opt: 1405, E(): 9.3e-84, (85.3% identity in 238 aa overlap); etc. |
| Functional category | Insertion seqs and phages |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4301563 | 4302789 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3827c|Rv3827c
LMARFEVPEGWCVQAFRFTLDPTEDQARALARHFGARRKAYNWAVATLKADIEAWRVTGIGTVKPSLRVLRKRWNTVKDEVCVNAETGAVWWPECSKEAYADGIGGAVDAYWNWQNSRSGKREGKTMGFPRFKKKGRDQDRVTFTTGAMRVEPDRRHLTLPVVGTVRTHENTRRIERLIATGRARVLAISVRRNGTRLDASVRVLVQRPQQPNVAQPGSRVGVDVGVRRLATVANEAGAVLEEVPNPRPLDTALKELRYASRARSRCTKGSRRYRERTTEISRLHRRVNDVRTHHLHVLTTRLAQTHGHIVVEGLDAAGMLRQKGLPGARARRRGLSDSALGTPRRHLSYKTGWYGSALVVADRWFPSLSVEPTVRPGLARLVAVKRGREAAAWLPNNPETGCKSRDH
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
