Gene Rv3868
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | ESX conserved component EccA1. ESX-1 type VII secretion system protein. |
| Comments | Rv3868, (MTV027.03), len: 573 aa. EccA1, esx conserved component, ESX-1 type VII secretion system protein. Member of the CbxX/CfqX family of hypothetical proteins; C-terminal end is highly similar to many e.g. P40118|CBXC_ALCEU|CBXXC|CFXXC CbxX protein (317 aa) FASTA scores: opt: 572, E(): 3e-24, (42.7% identity in 294 aa overlap); CAC48589 probable CBBX protein from Rhizobium meliloti (Sinorhizobium meliloti) plasmid pSymB (311 aa) FASTA scores: opt: 569, E(): 4.3e-24, (40.05% identity in 292 aa overlap); P95648|CBBX_RHOSH CBBX protein from Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides) (309 aa), FASTA scores: opt: 559, E(): 1.5e-23, (41.4% identity in 290 aa overlap); etc. Equivalent to O33089|Y2G8_MYCLE|ML0055|MLCB628.18c hypothetical 62.3 KDA protein from Mycobacterium leprae (573 aa), FASTA scores: opt: 3330, E(): 3.9e-175, (89.2% identity in 573 aa overlap); and similar to Q9CD28|Y282_MYCLE|ML2537 hypothetical 69.1 KDA protein from Mycobacterium leprae (640 aa), FASTA scores: opt: 943, E(): 2.4e-44, (37.5% identity in 571 aa overlap). Also similar to many proteins from Mycobacterium tuberculosis (strains H37Rv and CDC1551) e.g. O53687|Y282_MYCTU|Rv0282|MT0295|MTV035.10 hypothetical 68.1 KDA protein (631 aa), FASTA scores: opt: 936, E(): 5.8e-44, (39.05% identity in 568 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Transcriptomics | mRNA identified by microarray analysis and down-regulated after 4h of starvation (see Transcriptome citation). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Required for survival in primary murine macrophages, by transposon site hybridization (TraSH) in H37Rv (See Rengarajan et al., 2005). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4343314 | 4345035 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3868|eccA1
MTDRLASLFESAVSMLPMSEARSLDLFTEITNYDESACDAWIGRIRCGDTDRVTLFRAWYSRRNFGQLSGSVQISMSTLNARIAIGGLYGDITYPVTSPLAITMGFAACEAAQGNYADAMEALEAAPVAGSEHLVAWMKAVVYGAAERWTDVIDQVKSAGKWPDKFLAGAAGVAHGVAAANLALFTEAERRLTEANDSPAGEACARAIAWYLAMARRSQGNESAAVALLEWLQTTHPEPKVAAALKDPSYRLKTTTAEQIASRADPWDPGSVVTDNSGRERLLAEAQAELDRQIGLTRVKNQIERYRAATLMARVRAAKGMKVAQPSKHMIFTGPPGTGKTTIARVVANILAGLGVIAEPKLVETSRKDFVAEYEGQSAVKTAKTIDQALGGVLFIDEAYALVQERDGRTDPFGQEALDTLLARMENDRDRLVVIIAGYSSDIDRLLETNEGLRSRFATRIEFDTYSPEELLEIANVIAAADDSALTAEAAENFLQAAKQLEQRMLRGRRALDVAGNGRYARQLVEASEQCRDMRLAQVLDIDTLDEDRLREINGSDMAEAIAAVHAHLNMRE
Bibliography
- Gey Van Pittius NC, Gamieldien J, Hide W, Brown GD, Siezen RJ and Beyers AD [2001]. The ESAT-6 gene cluster of Mycobacterium tuberculosis and other high G+C Gram-positive bacteria. Secondary
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM and Rubin EJ [2003]. Genetic requirements for mycobacterial survival during infection. Mutant
- Rengarajan J et al. [2005]. Genome-wide requirements for Mycobacterium tuberculosis adaptation and survival in macrophages. Mutant
- [2009]. Systematic genetic nomenclature for type VII secretion systems. Nomenclature
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
