Gene Rv3870 (snm1)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown |
| Product | ESX conserved component EccCa1. ESX-1 type VII secretion system protein. Possible transmembrane protein. |
| Comments | Rv3870, (MTV027.05), len: 747 aa. EccCa1, esx conserved component, ESX-1 type VII secretion system protein, possible transmembrane protein, equivalent to O33087|ML0053|MLCB628.16c putative membrane protein from Mycobacterium leprae (744 aa), FASTA scores: opt: 4333, E(): 0, (85.4% identity in 746 aa overlap); and similar to N-terminal end of others e.g. Q9CD30|ML2535 hypothetical protein from Mycobacterium leprae (1329 aa), FASTA scores: opt: 1003, E(): 1e-52, (33.65% identity in 725 aa overlap); O86653|SC3C3.20c ATP/GTP binding protein from Streptomyces coelicolor (1321 aa), FASTA scores: opt: 1078, E(): 3e-57, (35.4% identity in 774 aa overlap); P71068|YUKA YUKA protein from Bacillus subtilis (1207 aa) FASTA scores: opt: 529, E(): 4.3e-24, (26.1% identity in 636 aa overlap); Q9KE81|BH0975 hypothetical protein from Bacillus halodurans (1489 aa), FASTA scores: opt: 455, E(): 1.5e-19, (27.1% identity in 734 aa overlap); etc. Also similar to N-terminal end of hypothetical proteins from Mycobacterium tuberculosis e.g. O53689|Rv0284|MTV035.12 (1330 aa), FASTA scores: opt: 982, E(): 1.9e-51, (33.8% identity in 719 aa overlap); O06264|Rv3447c|MTCY77.19c (1236 aa), FASTA scores: opt: 761, E(): 4.1e-38, (38.2% identity in 746 aa overlap); O53935|Rv1784|MTV049.06 (932 aa), FASTA scores: opt: 547, E(): 2.8e-25, (36.25% identity in 276 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop). Note some similarity (with hypothetical proteins from Mycobacterium tuberculosis and P71068|YUKA) continues in downstream ORF MTV027.06. |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Transcriptomics | mRNA identified by microarray analysis and down-regulated after 4h of starvation (see transcriptome citation). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). In THP-1 cells, M. tuberculosis H37Rv Rv3870 mutant is attenuated for growth and cytotoxicity; in C57BL/6 mouse lungs, growth and histopathology are attenuated; Rv3875 protein not detected by Western blot in culture filtrate of mutant; Rv3874 protein levels in cell pellet are comparable to wild-type but are reduced in culture filtrate, by ELISPOT (See Guinn et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4346481 | 4348724 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3870|eccCa1
MTTKKFTPTITRGPRLTPGEISLTPPDDLGIDIPPSGVQKILPYVMGGAMLGMIAIMVAGGTRQLSPYMLMMPLMMIVMMVGGLAGSTGGGGKKVPEINADRKEYLRYLAGLRTRVTSSATSQVAFFSYHAPHPEDLLSIVGTQRQWSRPANADFYAATRIGIGDQPAVDRLLKPAVGGELAAASAAPQPFLEPVSHMWVVKFLRTHGLIHDCPKLLQLRTFPTIAIGGDLAGAAGLMTAMICHLAVFHPPDLLQIRVLTEEPDDPDWSWLKWLPHVQHQTETDAAGSTRLIFTRQEGLSDLAARGPHAPDSLPGGPYVVVVDLTGGKAGFPPDGRAGVTVITLGNHRGSAYRIRVHEDGTADDRLPNQSFRQVTSVTDRMSPQQASRIARKLAGWSITGTILDKTSRVQKKVATDWHQLVGAQSVEEITPSRWRMYTDTDRDRLKIPFGHELKTGNVMYLDIKEGAEFGAGPHGMLIGTTGSGKSEFLRTLILSLVAMTHPDQVNLLLTDFKGGSTFLGMEKLPHTAAVVTNMAEEAELVSRMGEVLTGELDRRQSILRQAGMKVGAAGALSGVAEYEKYRERGADLPPLPTLFVVVDEFAELLQSHPDFIGLFDRICRVGRSLRVHLLLATQSLQTGGVRIDKLEPNLTYRIALRTTSSHESKAVIGTPEAQYITNKESGVGFLRVGMEDPVKFSTFYISGPYMPPAAGVETNGEAGGPGQQTTRQAARIHRFTAAPVLEEAPTP
Bibliography
- Gey Van Pittius NC, Gamieldien J, Hide W, Brown GD, Siezen RJ and Beyers AD [2001]. The ESAT-6 gene cluster of Mycobacterium tuberculosis and other high G+C Gram-positive bacteria. Secondary Phylogeny
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Sassetti CM and Rubin EJ [2003]. Genetic requirements for mycobacterial survival during infection. Mutant
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Guinn KM et al. [2004]. Individual RD1-region genes are required for export of ESAT-6/CFP-10 and for virulence of Mycobacterium tuberculosis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- [2009]. Systematic genetic nomenclature for type VII secretion systems. Nomenclature
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
