Gene Rv3872
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | PE family-related protein PE35 |
| Comments | Rv3872, (MTV027.07), len: 99 aa. PE35, Some similarity to Mycobacterium tuberculosis conserved PE family proteins (see Brennan & Delogu 2002), e.g. O69713|Rv3746c|MTV025.094c (111 aa), FASTA scores: opt: 306, E(): 5.5e-13, (50.5% identity in 99 aa overlap). Equivalent to AAK48354 from Mycobacterium tuberculosis strain CDC1551 (112 aa) but shorter 14 aa. |
| Functional category | Pe/ppe |
| Proteomics | Identified in culture filtrates of M. tuberculosis H37Rv (See Fortune et al., 2005; Malen et al., 2007). Identified by mass spectrometry in the culture filtrate of M. tuberculosis H37Rv but not the membrane protein fraction or whole cell lysates (See de Souza et al., 2011). |
| Transcriptomics | mRNA identified by microarray analysis and down-regulated after 4h of starvation (see Betts et al., 2002). mRNA identified by RT-PCR (See Amoudy et al., 2006). |
| Mutant | Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Survival of SCID mice infected with M. tuberculosis H37Rv Rv3872-Rv3873 mutant is comparable to those infected with wild-type; Rv3875 protein detected by Western blot in culture filtrate of mutant (See Hsu et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4350745 | 4351044 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3872|PE35
MEKMSHDPIAADIGTQVSDNALHGVTAGSTALTSVTGLVPAGADEVSAQAATAFTSEGIQLLASNASAQDQLHRAGEAVQDVARTYSQIDDGAAGVFAE
Bibliography
- Gey Van Pittius NC, Gamieldien J, Hide W, Brown GD, Siezen RJ and Beyers AD [2001]. The ESAT-6 gene cluster of Mycobacterium tuberculosis and other high G+C Gram-positive bacteria. Secondary
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Brennan MJ et al. [2002]. The PE multigene family: a 'molecular mantra' for mycobacteria. Review
- Hsu T et al. [2003]. The primary mechanism of attenuation of bacillus Calmette-Guerin is a loss of secreted lytic function required for invasion of lung interstitial tissue. Mutant
- Sassetti CM and Rubin EJ [2003]. Genetic requirements for mycobacterial survival during infection. Mutant
- Fortune SM et al. [2005]. Mutually dependent secretion of proteins required for mycobacterial virulence. Proteomics
- Amoudy HA et al. [2006]. Identification of transcriptionally active open reading frames within the RD1 genomic segment of Mycobacterium tuberculosis. Transcriptome
- Målen H et al. [2007]. Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
