Gene Rv3884c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | ESX conserved component EccA2. ESX-2 type VII secretion system protein. Probable CbxX/CfqX family protein. |
| Comments | Rv3884c, (MTCY15F10.28), len: 619 aa. eccA2, esx conserved component, ESX-2 type VII secretion system protein. Probable CbxX/CfqX protein family, similar to hypothetical proteins from Mycobacterium leprae e.g. Q9CD28|Y282_MYCLE|ML2537 (640 aa), FASTA scores: opt: 725, E(): 2.9e-34, (28.95% identity in 587 aa overlap); O33089|Y2G8_MYCLE|ML0055|MLCB628.18c (belongs to the CbxX/CfqX family) (573 aa); Q9CBV5|ML1536 (610 aa) FASTA scores: opt: 648, E(): 7.4e-30, (31.5% identity in 549 aa overlap). Also similar to proteins belonging to the CbxX/CfqX family e.g. Q9RKZ2|SC6D7.05c putative CbxX/CfqX family protein from Streptomyces coelicolor (618 aa) FASTA scores: opt: 557, E(): 1.3e-24, (28.6% identity in 601 aa overlap); P27643|SP5K_BACSU|SPOVK|SPOVJ stage V sporulation protein K from Bacillus subtilis (322 aa) FASTA scores: opt: 485, E(): 1.1e-20, (35.0% identity in 280 aa overlap) (similarity only at C-terminus); Q9KAC6|BH2363 stage V sporulation protein K from Bacillus halodurans (315 aa), FASTA scores: opt: 462, E(): 2.2e-19, (36.05% identity in 244 aa overlap) (similarity only at C-terminus); etc. And similar to hypothetical proteins from Mycobacterium tuberculosis belonging to the CbxX/CfqX family e.g. O53687|Y282_MYCTU|Rv0282|MT0295|MTV035.10 hypothetical 68.1 KDA protein (631 aa), FASTA scores: opt: 743, E(): 2.6e-35, (29.9% identity in 612 aa overlap); O69733|Y2G8_MYCTU|Rv3868|MT3981|MTV027.03 hypothetical 62.4 KDA protein (573 aa), FASTA scores: opt: 678, E(): 1.3e-31, (31.25% identity in 589 aa overlap); O53947|YH98_MYCTU|Rv1798|MT1847|MTV049.20 (610 aa) FASTA scores: opt: 669, E(): 4.6e-31, (30.95% identity in 549 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Seems to belong to the CbxX/CfqX family. |
| Functional category | Cell wall and cell processes |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4364979 | 4366838 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3884c|eccA2
MSRMVDTMGDLLTARRHFDRAMTIKNGQGCVAALPEFVAATEADPSMADAWLGRIACGDRDLASLKQLNAHSEWLHRETTRIGRTLAAEVQLGPSIGITVTDASQVGLALSSALTIAGEYAKADALLANRELLDSWRNYQWHQLARAFLMYVTQRWPDVLSTAAEDLPPQAIVMPAVTASICALAAHAAAHLGQGRVALDWLDRVDVIGHSRSSERFGADVLTAAIGPADIPLLVADLAYVRGMVYRQLHEEDKAQIWLSKATINGVLTDAAKEALADPNLRLIVTDERTIASRSDRWDASTAKSRDQLDDDNAAQRRGELLAEGRELLAKQVGLAAVKQAVSALEDQLEVRMMRLEHGLPVEGQTNHMLLVGPPGTGKTTTAEALGKIYAGMGIVRHPEIREVRRSDFCGHYIGESGPKTNELIEKSLGRIIFMDEFYSLIERHQDGTPDMIGMEAVNQLLVQLETHRFDFCFIGAGYEDQVDEFLTVNPGLAGRFNRKLRFESYSPVEIVEIGHRYATPRASQLDDAAREVFLDAVTTIRNYTTPSGQHGIDAMQNGRFARNVIERAEGFRDTRVVAQKRAGQPVSVQDLQIITATDIDAAIRSVCSDNRDMAAIVW
Bibliography
- Gey Van Pittius NC, Gamieldien J, Hide W, Brown GD, Siezen RJ and Beyers AD [2001]. The ESAT-6 gene cluster of Mycobacterium tuberculosis and other high G+C Gram-positive bacteria. Secondary
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- [2009]. Systematic genetic nomenclature for type VII secretion systems. Nomenclature
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
