Gene Rv3895c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown |
| Product | ESX conserved component EccB2. ESX-2 type VII secretion system protein. Probable membrane protein. |
| Comments | Rv3895c, (MTCY15F10.17), len: 495 aa. EccB2, esx conserved component, ESX-2 type VII secretion system protein, probable membrane protein, highly similar to two conserved membrane protein from Mycobacterium leprae: Q9Z5I3|ML1544|MLCB596.27 (506 aa), FASTA scores: opt: 1070, E(): 1.4e-53, (39.8% identity in 485 aa overlap); and Q9CD29|ML2536 (552 aa), FASTA scores: opt: 483, E(): 4e-20, (36.85% identity in 499 aa overlap). Also highly similar to various proteins from Mycobacterium tuberculosis e.g. O53933|Rv1782|MTV049.04 hypothetical protein (506 aa), FASTA scores: opt: 1106, E(): 1.2e-55, (41.25% identity in 485 aa overlap); O69734|Rv3869|MTV027.04 hypothetical protein (480 aa), FASTA scores: opt: 795, E(): 6.1e-38, (36.0% identity in 486 aa overlap); O33088|ML0054|MLCB628.17c putative membrane protein (481 aa), FASTA scores: opt: 740, E(): 8.3e-35, (35.65% identity in 485 aa overlap); etc. |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4380453 | 4381940 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3895c|eccB2
MPLSLSNRDQNSGHLFYNRRLRAATTRFSVRMKHDDRKQTAALALSMVLVAIAAGWMMLLNVLKPTGIVGDSAIIGDRDSGALYARIDGRLYPALNLTSARLATGTAGQPTWVKPAEIAKYPTGPLVGIPGAPAAMPVNRGAVSAWAVCDTAGRPRSADKPVVTSIAGPITGGGRATHLRDDAGLLVTFDGSTYVIWGGKRSQIDPTNRAVTLSLGLDPGVTSPIQISRALFDGLPATEPLRVPAVPEAGTPSTWVPGARVGSVLQAQTAGGGSQFYVLLPDGVQKISSFVADLLRSANSYGAAAPRVVTPDVLVHTPQVTSLPVEYYPAGRLNFVDTAADPTTCVSWEKASTDPQARVAVYNGRGLPVPPSMDSRIVRLVRDDRAPASVVATQVLVLPGAANFVTSTSGVITAESRESLFWVSGNGVRFGIANDEATLRALGLDPGAAVQAPWPLLRTFAAGPALSRDAALLARDTVPTLGQVAIVTTTAKAGA
Bibliography
- Gey Van Pittius NC, Gamieldien J, Hide W, Brown GD, Siezen RJ and Beyers AD [2001]. The ESAT-6 gene cluster of Mycobacterium tuberculosis and other high G+C Gram-positive bacteria. Secondary Phylogeny
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- [2009]. Systematic genetic nomenclature for type VII secretion systems. Nomenclature
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
