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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionFunction unknown; probably involved in cellular metabolism.
ProductProbable peptidoglycan hydrolase
CommentsRv3915, (MTV028.06), len: 406 aa. Probable peptidoglycan hydrolase, equivalent to Q9CCX8|ML2704 putative hydrolase from Mycobacterium leprae (406 aa) FASTA scores: opt: 2341, E(): 2.7e-138, (86.95% identity in 406 aa overlap); the N-terminal end is highly similar to Q59535 N-acetymuramyl-L-alanine amidase from Mycobacterium leprae (205 aa), FASTA scores: opt: 1046, E(): 5.7e-58, (84.85% identity in 185 aa overlap). Also similar to other hydrolases (especially amidases) e.g. C-terminal end of Q9K6R3|LYTC|BH3665 N-acetylmuramoyl-L-alanine amidase (major autolysin) from Bacillus halodurans (588 aa), FASTA scores: opt: 363, E(): 4.3e-15, (33.15% identity in 356 aa overlap); Q9PKC7|TC0539 putative N-acetylmuramoyl-L-alanine amidase from Chlamydia muridarum (268 aa), FASTA scores: opt: 285, E(): 1.6e-10, (26.05% identity in 242 aa overlap) (RV3915 product appears longer 127 aa); Q9S596|PDCA penicillin-resistant DD-carboxypeptidase from Myxococcus xanthus (302 aa), FASTA scores: opt: 270, E(): 1.5e-09, (39.85% identity in 158 aa overlap); etc. Note that previously known as cwlM. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007).
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010).
MutantEssential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS44031924404412+
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3915|Rv3915
MPSPRREDGDALRCGDRSAAVTEIRAALTALGMLDHQEEDLTTGRNVALELFDAQLDQAVRAFQQHRGLLVDGIVGEATYRALKEASYRLGARTLYHQFGAPLYGDDVATLQARLQDLGFYTGLVDGHFGLQTHNALMSYQREYGLAADGICGPETLRSLYFLSSRVSGGSPHAIREEELVRSSGPKLSGKRIIIDPGRGGVDHGLIAQGPAGPISEADLLWDLASRLEGRMAAIGMETHLSRPTNRSPSDAERAATANAVGADLMISLRCETQTSLAANGVASFHFGNSHGSVSTIGRNLADFIQREVVARTGLRDCRVHGRTWDLLRLTRMPTVQVDIGYITNPHDRGMLVSTQTRDAIAEGILAAVKRLYLLGKNDRPTGTFTFAELLAHELSVERAGRLGGS