Gene Rv3916c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved hypothetical protein |
| Comments | Rv3916c, (MTV028.07c), len: 244 aa. Conserved hypothetical protein, equivalent to Q50200|ML2705|L222-ORF1 hypothetical protein from Mycobacterium leprae (259 aa), FASTA scores: opt: 1266, E(): 2e-74, (76.4% identity in 250 aa overlap). Also highly similar (but with gaps) to Q9R3S2|STH24.10 hypothetical 22.6 KDA protein from Streptomyces coelicolor (205 aa), FASTA scores: opt: 387, E(): 7.5e-18, (40.25% identity in 231 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Transposon mutant is hypersensitive to acidified nitrite (See Darwin et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4404433 | 4405167 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3916c|Rv3916c
VSARITALRLEAFEQLPKHARRCVFWEVDPAILGKDDHLADPEFEKEAWLSMVMLEWGSCGQVATAVPDERSHAEPPCLGYVLYAPPSAVPRAQRFPTAPVSADAVLLTSMGIERGQADDDLPHSLIARVIEELVRRGVRALEAFGRTPAATDLQNPGAVTPDVRPVLEALGDCCVEHCIIDANFLMDVGFVVVAPHPYFPRLRLELDKGLGWKAEVEAALERLLENARLQEPIAAGSTAGNTS
Bibliography
- Darwin KH et al. [2003]. The proteasome of Mycobacterium tuberculosis is required for resistance to nitric oxide. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Song H, Sandie R, Wang Y, Andrade-Navarro MA and Niederweis M [2008]. Identification of outer membrane proteins of Mycobacterium tuberculosis. Localization
- Mazandu GK et al. [2012]. Function prediction and analysis of mycobacterium tuberculosis hypothetical proteins. Function
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
