Gene Rv3917c (parA)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in chromosome partition. Localize to both POLES of the predivisional cell following completion of DNA replication. Binds to the DNA origin of replication. |
| Product | Probable chromosome partitioning protein ParB |
| Comments | Rv3917c, (MTV028.08c, MT4036), len: 344 aa. Probable parB, chromosome partitioning protein, equivalent to Q50201|PARB_MYCLE|ML2706 probable chromosome partitioning protein from Mycobacterium leprae (333 aa), FASTA scores: opt: 1654, E(): 1.6e-88, (78.6% identity in 332 aa overlap). Also highly similar to to others e.g. Q9S6U1|STH24.09 putative partitioning or sporulation protein from Streptomyces coelicolor (328 aa), FASTA scores: opt: 966, E(): 9.7e-49, (58.55% identity in 287 aa overlap) (no similarity on N-terminus); Q9PB63|PARB_XYLFA|XF2281 probable chromosome partitioning protein from Xylella fastidiosa (310 aa), FASTA scores: opt: 598, E(): 1.8e-27, (38.65% identity in 326 aa overlap); P31857|PARB_PSEPU probable chromosome partitioning protein from Pseudomonas putida (290 aa), FASTA scores: opt: 573, E(): 4.6e-26, (40.35% identity in 322 aa overlap); etc. Contains probable helix-turn-helix motif at aa 179 to 200 (Score 1150, +3.1 0 SD). Belongs to the ParB family. Note that previously known as parA. |
| Functional category | Cell wall and cell processes |
| Proteomics | Predicted secreted protein - identified in culture filtrates of M. tuberculosis H37Rv; signal peptide predicted (See Malen et al., 2007). Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Transcriptomics | mRNA identified by microarray analysis and down-regulated after 4h, 24h and 96h of starvation (see citation below). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4405457 | 4406491 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3917c|parB
MTQPSRRKGGLGRGLAALIPTGPADGESGPPTLGPRMGSATADVVIGGPVPDTSVMGAIYREIPPSAIEANPRQPRQVFDEEALAELVHSIREFGLLQPIVVRSLAGSQTGVRYQIVMGERRWRAAQEAGLATIPAIVRETGDDNLLRDALLENIHRVQLNPLEEAAAYQQLLDEFGVTHDELAARIGRSRPLITNMIRLLKLPIPVQRRVAAGVLSAGHARALLSLEAGPEAQEELASRIVAEGLSVRATEETVTLANHEANRQAHHSDATTPAPPRRKPIQMPGLQDVAERLSTTFDTRVTVSLGKRKGKIVVEFGSVDDLARIVGLMTTDGRDKGLHRDAL
Bibliography
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2007]. Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
