Gene Mb3698c
in Mycobacterium bovis AF2122/97
General annotation
| Type | CDS |
| Function | Unknown |
| Product | PROBABLE ENDONUCLEASE III NTH (DNA-(APURINIC OR APYRIMIDINIC SITE)LYASE) (AP LYASE) (AP ENDONUCLEASE CLASS I) (ENDODEOXYRIBONUCLEASE (APURINIC OR APYRIMIDINIC)) (DEOXYRIBONUCLEASE (APURINIC OR APYRIMIDINIC)) |
| Comments | Mb3698c, nth, len: 245 aa. Equivalent to Rv3674c,len: 245 aa, from Mycobacterium tuberculosis strain H37Rv,(100.0% identity in 245 aa overlap). Probable nth,endonuclease III (EC 4.2.99.18), equivalent to Q9CB92|NTH|ML2301 PUTATIVE ENDONUCLEASE III from Mycobacterium leprae (272 aa), FASTA scores: opt: 1363,E(): 3.6e-81, (89.4% identity in 226 aa overlap). Also similar to many e.g. Q9XA44|SCH17.03c from Streptomyces coelicolor (250 aa), FASTA scores: opt: 937, E(): 2.2e-55,(61.65% identity in 219 aa overlap); P46303|UVEN_MICLU from Micrococcus luteus (Micrococcus lysodeikticus) (279 aa), FASTA scores: opt: 899, E(): 8.1e-53, (58.45% identity in 248 aa overlap); P73715|END3_SYNY3|NTH|SLR1822 from Synechocystis sp. strain PCC 6803 (219 aa), FASTA scores: opt: 684, E(): 1.7e-38, (52.2% identity in 203 aa overlap); P39788|END3_BACSU|NTH|JOOB from Bacillus subtilis (219 aa), FASTA scores: opt: 552, E(): 1.2e-29,(43.3% identity in 194 aa overlap); etc. Equivalent to AAK48142 from Mycobacterium tuberculosis strain CDC1551 (262 aa) but shorter 17 aa. Contains PS00764 Endonuclease III iron-sulfur binding region signature, and PS01155 Endonuclease III family signature. BELONGS TO THE NTH/MUTY FAMILY. COFACTOR: BINDS A 4FE-4S CLUSTER WHICH IS NOT IMPORTANT FOR THE CATALYTIC ACTIVITY, BUT WHICH IS PROBABLY INVOLVED IN THE PROPER POSITIONING OF THE ENZYME ALONG THE DNA STRAND (BY SIMILARITY). N-terminus extended since first submission (previously 226 aa). |
| Functional category | |
| Mutant | Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4052504 | 4053241 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium bovis AF2122-97|Mb3698c|nth
MPGRWSAETRLALVRRARRMNRALAQAFPHVYCELDFTTPLELAVATILSAQSTDKRVNLTTPALFARYRTARDYAQADRTELESLIRPTGFYRNKAASLIGLGQALVERFGGEVPATMDKLVTLPGVGRKTANVILGNAFGIPGITVDTHFGRLVRRWRWTTAEDPVKVEQAVGELIERKEWTLLSHRVIFHGRRVCHARRPACGVCVLAKDCPSFGLGPTEPLLAAPLVQGPETDHLLALAGL
Bibliography
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