Gene Rv3674c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand (oxidized pyrimidines), 5' from the damaged site [catalytic activity: endonucleolytic cleavage near apurinic or apyrimidinic sites to products with 5'-phosphate]. |
| Product | Probable endonuclease III Nth (DNA-(apurinic or apyrimidinic site)lyase) (AP lyase) (AP endonuclease class I) (endodeoxyribonuclease (apurinic or apyrimidinic)) (deoxyribonuclease (apurinic or apyrimidinic)) |
| Comments | Rv3674c, (MT3775, MTV025.022c), len: 245 aa. Probable nth, endonuclease III (see citation below), equivalent to Q9CB92|nth|ML2301 putative endonuclease III from Mycobacterium leprae (272 aa), FASTA scores: opt: 1363, E(): 3.6e-81, (89.4% identity in 226 aa overlap). Also similar to many e.g. Q9XA44|SCH17.03c from Streptomyces coelicolor (250 aa), FASTA scores: opt: 937, E(): 2.2e-55, (61.65% identity in 219 aa overlap); P46303|UVEN_MICLU from Micrococcus luteus (Micrococcus lysodeikticus) (279 aa), FASTA scores: opt: 899, E(): 8.1e-53, (58.45% identity in 248 aa overlap); P73715|END3_SYNY3|nth|SLR1822 from Synechocystis sp. strain PCC 6803 (219 aa), FASTA scores: opt: 684, E(): 1.7e-38, (52.2% identity in 203 aa overlap); P39788|END3_BACSU|nth|JOOB from Bacillus subtilis (219 aa), FASTA scores: opt: 552, E(): 1.2e-29, (43.3% identity in 194 aa overlap); etc. Equivalent to AAK48142 from Mycobacterium tuberculosis strain CDC1551 (262 aa) but shorter 17 aa. Contains PS00764 Endonuclease III iron-sulfur binding region signature, and PS01155 Endonuclease III family signature. Belongs to the nth/MUTY family. Cofactor: binds a 4FE-4S cluster which is not important for the catalytic activity, but which is probably involved in the proper positioning of the enzyme along the DNA strand (by similarity). N-terminus extended since first submission (previously 226 aa). |
| Functional category | Information pathways |
| Proteomics | Identified in the cell wall and cell membrane fractions of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4115157 | 4115894 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3674c|nth
VPGRWSAETRLALVRRARRMNRALAQAFPHVYCELDFTTPLELAVATILSAQSTDKRVNLTTPALFARYRTARDYAQADRTELESLIRPTGFYRNKAASLIGLGQALVERFGGEVPATMDKLVTLPGVGRKTANVILGNAFGIPGITVDTHFGRLVRRWRWTTAEDPVKVEQAVGELIERKEWTLLSHRVIFHGRRVCHARRPACGVCVLAKDCPSFGLGPTEPLLAAPLVQGPETDHLLALAGL
Bibliography
- Mizrahi V et al. [1998]. DNA repair in Mycobacterium tuberculosis. What have we learnt from the genome sequence? Secondary Function
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
