Gene Mb3913c
in Mycobacterium bovis AF2122/97
General annotation
| Type | CDS |
| Function | Unknown |
| Product | membrane-anchored mycosin mycp1 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-1) |
| Comments | Mb3913c, -, len: 446 aa. Equivalent to Rv3883c,len: 446 aa, from Mycobacterium tuberculosis strain H37Rv,(99.8% identity in 446 aa overlap). Possible secreted protease (EC 3.4.-.-), equivalent to O33076|ML0041|MLCB628.04 PROBABLE SECRETED PROTEASE from Mycobacterium leprae (446 aa), FASTA scores: opt: 2448,E(): 1.5e-124, (79.15% identity in 446 aa overlap); and highly similar, but in part, to several putative proteases from Mycobacterium leprae; Q9CBV3|ML1538 (567 aa) FASTA scores: opt: 902, E(): 3e-41, (37.25% identity in 556 aa overlap); and Q9CD36|ML2528 (475 aa), FASTA scores: opt: 873, E(): 9.4e-40, (42.7% identity in 459 aa overlap). Shows also similarity with several proteases from other organisms e.g. Q9PCD0|XF1851 SERINE PROTEASE from Xylella fastidiosa (1000 aa), FASTA scores: opt: 281, E(): 1.3e-07, (27.95% identity in 422 aa overlap); P42780|BPRX_BACNO EXTRACELLULAR SUBTILISIN-LIKE PROTEASE PRECURSOR (EC 3.4.21.-) from Bacteroides nodosus (Dichelobacter nodosus) (595 aa), FASTA scores: opt: 270,E(): 3.2e-07, (28.9% identity in 384 aa overlap); Q46541|APRV5 ACIDIC PROTEASE V5 from Bacteroides nodosus (Dichelobacter nodosus) (595 aa), FASTA scores: opt: 264,E(): 6.8e-07, (28.65% identity in 384 aa overlap); etc. Also highly similar to various proteins from Mycobacterium tuberculosis e.g. O53695|Rv0291|MTV035.19 PUTATIVE PROTEASE (461 aa), FASTA scores: opt: 1168, E(): 1.2e-55,(44.6% identity in 453 aa overlap); O53945|Rv1796|MTV049.18 HYPOTHETICAL 60.0 KDA PROTEIN (585 aa), FASTA scores: opt: 928, E(): 1.2e-42, (37.85% identity in 555 aa overlap) (note gap from aa 155-264); and downstream ORF O05458|Rv3886c|MTCY15F10.26 HYPOTHETICAL 55.6 KDA PROTEIN (550 aa), FASTA scores: opt: 910, E(): 1.1e-41, (40.15% identity in 533 aa overlap) (note partial gap from aa 146-234); etc. Equivalent to AAK48366 from Mycobacterium tuberculosis strain CDC1551 (411 aa) but longer 35 aa. Has signal sequence with possible signal peptidase I cleavage site in residues 19-21 (ASA) and hydrophobic stretch at C-terminus,followed by short positively charged segment, that could act as membrane anchor. |
| Functional category | Intermediary metabolism and respiration |
| Mutant | Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4299782 | 4301122 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium bovis AF2122-97|Mb3913c|mycp1
MHRIFLITVALALLTASPASAITPPPIDPGALPPDVAGPDQPTEQRVLCASPTTLPGSGFHDPPWSNTYLGVADAHKFATGAGVTVAVIDTGVDASPRVPAEPGGDFVDQAGNGLSDCDAHGTLTASIIAGRPAPTDGFVGVAPDARLLSLRQTSEAFEPVGSQANPNDPNATPAAGSIRSLARAVVHAANLGVGVINISEAACYKVSRPIDETSLGASIDYAVNVKGVVVVVAAGNTGGDCVQNPAPDPSTPGDPRGWNNVQTVVTPAWYAPLVLSVGGIGQTGMPSSFSMHGPWVDVAAPAENIVALGDTGEPVNALQGREGPVPIAGTSFAAAYVSGLAALLRQRFPDLTPAQIIHRITATARHPGGGVDDLVGAGVIDAVAALTWDIPPGPASAPYNVRRLPPPVVEPGPDRRPITAVALVAVGLTLALGLGALARRALSRR
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