Gene Rv3883c (snm8)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Has proteolytic activity. Cleaves ESPB|Rv3881c. Expressed during infection of macrophages. |
| Product | Membrane-anchored mycosin MycP1 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-1) |
| Comments | Rv3883c, (MTCY15F10.29), len: 446 aa. MycP1, membrane-anchored serine protease (mycosin) (see citations below), equivalent to O33076|ML0041|MLCB628.04 probable secreted protease from Mycobacterium leprae (446 aa), FASTA scores: opt: 2448, E(): 1.5e-124, (79.15% identity in 446 aa overlap); and highly similar, but in part, to several putative proteases from Mycobacterium leprae; Q9CBV3|ML1538 (567 aa) FASTA scores: opt: 902, E(): 3e-41, (37.25% identity in 556 aa overlap); and Q9CD36|ML2528 (475 aa), FASTA scores: opt: 873, E(): 9.4e-40, (42.7% identity in 459 aa overlap). Shows also similarity with several proteases from other organisms e.g. Q9PCD0|XF1851 serine protease from Xylella fastidiosa (1000 aa), FASTA scores: opt: 281, E(): 1.3e-07, (27.95% identity in 422 aa overlap); P42780|BPRX_BACNO extracellular subtilisin-like protease precursor from Bacteroides nodosus (Dichelobacter nodosus) (595 aa), FASTA scores: opt: 270, E(): 3.2e-07, (28.9% identity in 384 aa overlap); Q46541|APRV5 acidic protease V5 from Bacteroides nodosus (Dichelobacter nodosus) (595 aa), FASTA scores: opt: 264, E(): 6.8e-07, (28.65% identity in 384 aa overlap); etc. Also highly similar to various proteins from Mycobacterium tuberculosis e.g. O53695|Rv0291|MTV035.19 probable membrane-anchored mycosin MYCP3 (461 aa), FASTA scores: opt: 1168, E(): 1.2e-55, (44.6% identity in 453 aa overlap); O53945|Rv1796|MTV049.18 probable membrane-anchored mycosin MYCP5 (585 aa), FASTA scores: opt: 928, E(): 1.2e-42, (37.85% identity in 555 aa overlap) (note gap from aa 155-264); and downstream ORF O05458|Rv3886c|MTCY15F10.26 probable membrane-anchored mycosin MYCP2 (550 aa), FASTA scores: opt: 910, E(): 1.1e-41, (40.15% identity in 533 aa overlap) (note partial gap from aa 146-234); etc. Equivalent to AAK48366 from Mycobacterium tuberculosis strain CDC1551 (411 aa) but longer 35 aa. Has signal sequence with possible signal peptidase I cleavage site in residues 19-21 (ASA) and hydrophobic stretch at C-terminus, followed by short positively charged segment, that seems to act as a membrane anchor. Activated by Ca2+ (see Dave et al., 2002). Contains three serine protease, subtilase family active site motifs: a aspartic acid active site motif (PS00136); a histidine active site motif (PS00137); and a serine active site motif (PS00138). Belongs to peptidase family S8 (also known as the subtilase family), pyrolysin subfamily. Conserved in M. tuberculosis, M. leprae, M. bovis and M. avium paratuberculosis; predicted to be essential for in vivo survival and pathogenicity (See Ribeiro-Guimaraes and Pessolani, 2007). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). M. tuberculosis Erdman mycP1|Rv3883c mutant does not secrete EsxA|Rv3875 and complementation with mycP1-S332A results in increased secretion of ESX-1 substrates compared to wild-type; growth of mutant in BALB/c mice and in C57BL/6 bone marrow-derived macrophages is impaired but not when complemented with mycP1-S332A; BALB/c mice are not killed when infected with mutant or mutant complemented with mycP1-S332A (See Ohol et al., 2010). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 4363417 | 4364757 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3883c|mycP1
VHRIFLITVALALLTASPASAITPPPIDPGALPPDVTGPDQPTEQRVLCASPTTLPGSGFHDPPWSNTYLGVADAHKFATGAGVTVAVIDTGVDASPRVPAEPGGDFVDQAGNGLSDCDAHGTLTASIIAGRPAPTDGFVGVAPDARLLSLRQTSEAFEPVGSQANPNDPNATPAAGSIRSLARAVVHAANLGVGVINISEAACYKVSRPIDETSLGASIDYAVNVKGVVVVVAAGNTGGDCVQNPAPDPSTPGDPRGWNNVQTVVTPAWYAPLVLSVGGIGQTGMPSSFSMHGPWVDVAAPAENIVALGDTGEPVNALQGREGPVPIAGTSFAAAYVSGLAALLRQRFPDLTPAQIIHRITATARHPGGGVDDLVGAGVIDAVAALTWDIPPGPASAPYNVRRLPPPVVEPGPDRRPITAVALVAVGLTLALGLGALARRALSRR
Bibliography
- Brown GD, Dave JA, Gey Van Pittius NC, Stevens L, Ehlers MR and Beyers AD [2000]. The mycosins of Mycobacterium tuberculosis H37Rv: a family of subtilisin-like serine proteases. Product Localization
- Gey Van Pittius NC, Gamieldien J, Hide W, Brown GD, Siezen RJ and Beyers AD [2001]. The ESAT-6 gene cluster of Mycobacterium tuberculosis and other high G+C Gram-positive bacteria. Secondary Phylogeny
- Dave JA et al. [2002]. Mycosin-1, a subtilisin-like serine protease of Mycobacterium tuberculosis, is cell wall-associated and expressed during infection of macrophages. Product Biochemistry Localization Function
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Ribeiro-Guimarães ML et al. [2007]. Comparative genomics of mycobacterial proteases. Homology
- [2009]. Systematic genetic nomenclature for type VII secretion systems. Nomenclature
- Ohol YM et al. [2010]. Mycobacterium tuberculosis MycP1 protease plays a dual role in regulation of ESX-1 secretion and virulence. Function Mutant
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
