Gene Rv0491
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Transcriptional regulatory protein part of the two component regulatory system REGX3/SENX3. |
| Product | Two component sensory transduction protein RegX3 (transcriptional regulatory protein) (probably LuxR-family) |
| Comments | Rv0491, (MTCY20G9.17), len: 227 aa. RegX3, response regulator protein (sensory transduction protein) (see citations below), equivalent to O07130|RGX3_MYCBO|REGX3 sensory transduction protein from Mycobacterium bovis BCG (227 aa); AAG09797.1|AF258346_2|AF258346|REGX3 response regulator from Mycobacterium smegmatis (228 aa); equivalent to P54884|RGX3_MYCLE|REGX3 sensory transduction protein from Mycobacterium leprae (198 aa), FASTA scores : E(): 0, (95.4% identity in 197 aa overlap). Also highly similar to other response regulators e.g. AAG43239.1|AF123314_2 |AF123314 putative response regulator from Corynebacterium glutamicum (232 aa). |
| Functional category | Regulatory proteins |
| Proteomics | Proteomics shows upregulation in isoniazid-resistant M. tuberculosis clinical isolates (See Jiang et al., 2006). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Growth of M. tuberculosis Mt103 transposon mutant in BALB/c mice and in mouse bone marrow-derived macrophages is unaffected (See Ewann et al., 2002). M. tuberculosis H37Rv senX3-regX3 mutant has reduced ability to survive in THP-1 and bone-marrow-derived macrophages; mutant is attenuated in SCID and DBA/2 mice (See Parish et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 580809 | 581492 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0491|regX3
MTSVLIVEDEESLADPLAFLLRKEGFEATVVTDGPAALAEFDRAGADIVLLDLMLPGMSGTDVCKQLRARSSVPVIMVTARDSEIDKVVGLELGADDYVTKPYSARELIARIRAVLRRGGDDDSEMSDGVLESGPVRMDVERHVVSVNGDTITLPLKEFDLLEYLMRNSGRVLTRGQLIDRVWGADYVGDTKTLDVHVKRLRSKIEADPANPVHLVTVRGLGYKLEG
Bibliography
- Supply P, Magdalena J, Himpens S and Locht C [1997]. Identification of novel intergenic repetitive units in a mycobacterial two-component system operon. Sequence
- Himpens S et al. [2000]. Molecular characterization of the mycobacterial SenX3-RegX3 two-component system: evidence for autoregulation. Secondary Regulation
- Ewann F, Jackson M, Pethe K, Cooper A, Mielcarek N, Ensergueix D, Gicquel B, Locht C and Supply P [2002]. Transient requirement of the PrrA-PrrB two-component system for early intracellular multiplication of Mycobacterium tuberculosis. Mutant
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Parish T, Smith DA, Roberts G, Betts J and Stoker NG [2003]. The senX3-regX3 two-component regulatory system of Mycobacterium tuberculosis is required for virulence. Mutant Regulation
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Jiang X et al. [2006]. Comparison of the proteome of isoniazid-resistant and -susceptible strains of Mycobacterium tuberculosis. Proteomics
- King-Scott J, Nowak E, Mylonas E, Panjikar S, Roessle M, Svergun DI and Tucker PA [2007]. The structure of a full-length response regulator from Mycobacterium tuberculosis in a stabilized three-dimensional domain-swapped, activated state. Structure
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
