Gene Rv0586
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in transcriptional mechanism. |
| Product | Probable transcriptional regulatory protein Mce2R (GntR-family) |
| Comments | Rv0586, (MTCY19H5.36c), len: 240 aa. Probable mce2R, transcriptional regulator, GntR family, part of mce2 operon, similar to many e.g. P33233|LLDR_ECOLI putative L-lactate dehydrogenase operon regulatory protein from Escherichia coli (258 aa), FASTA scores: opt: 225, E(): 9.3e-08, (26.7% identity in 232 aa overlap); etc. Also similar to other M. tuberculosis transcriptional regulators GntR proteins e.g. Rv3060c, Rv0792c, etc. Contains PS00043 Bacterial regulatory proteins, gntR family signature and probable helix-turn helix motif from aa 35-56 (Score 1531, +4.40 SD). |
| Functional category | Regulatory proteins |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Operon | Rv0586 and Rv0587 are co-transcribed, by RT-PCR (See Santangelo et al., 2009). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 684410 | 685132 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0586|mce2R
MALQPVTRRSVPEEVFEQIATDVLTGEMPPGEALPSERRLAELLGVSRPAVREALKRLSAAGLVEVRQGDVTTVRDFRRHAGLDLLPRLLFRNGELDISVVRSILEARLRNFPKVAELAAERNEPELAELLQDSLRALDTEEDPIVWQRHTLDFWDHVVDSAGSIVDRLMYNAFRAAYEPTLAALTTTMTAAAKRPSDYRKLADAICSGDPTGAKKAAQDLLELANTSLMAVLVSQASRQ
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Vindal V et al. [2008]. Identification of operator sites within the upstream region of the putative mce2R gene from mycobacteria. Product
- Santangelo Mde L, Blanco F, Campos E, Soria M, Bianco MV, Klepp L, Alito A, Zabal O, Cataldi A and Bigi F [2009]. Mce2R from Mycobacterium tuberculosis represses the expression of the mce2 operon. Function Operon
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
